Selection for high-level resistance by chronic triclosan exposure is not universal

Andrew J. McBain; Ruth G. Ledder; Prem Sreenivasan; Peter Gilbert

doi:10.1093/jac/dkh168

Selection for high-level resistance by chronic triclosan exposure is not universal

Andrew J. McBain, Ruth G. Ledder, Prem Sreenivasan, Peter Gilbert

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives: To investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria. Methods: A gradient plate technique was used to expose Fusobacterium nucleatum, Lactobacillus rhamnosus, Neisseria subflava, Porphyromonas gingivalis, Actinomyces naeslundii, Prevotella nigrescens, Streptococcus oralis, Streptococcus sanguis, Streptococcus mutans and Veillonella dispar repeatedly to escalating, sublethal concentrations of triclosan. Escherichia coli ATCC 8739 was included as an organism showing the triclosan resistance development trait. MIC values towards chlorhexidine, metronidazole and tetracycline were determined before and after biocide exposure. Results: N. subflava, Pr. nigrescens Po. gingivalis and E. coli were highly susceptible to triclosan (MIC range 0.1-3.9 mg/L), whereas the lactobacillus and S. mutans were less susceptible (MIC range 15.6-20.8 mg/L). Triclosan exposure resulted in a highly significant (∼ 400-fold) reduction in triclosan susceptibility (P

Original language	English
Pages (from-to)	772-777
Number of pages	5
Journal	Journal of Antimicrobial Chemotherapy
Volume	53
Issue number	5
DOIs	https://doi.org/10.1093/jac/dkh168
Publication status	Published - May 2004

Keywords

Antibiotics
Dental plaque
Susceptibility

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10.1093/jac/dkh168

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title = "Selection for high-level resistance by chronic triclosan exposure is not universal",

abstract = "Objectives: To investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria. Methods: A gradient plate technique was used to expose Fusobacterium nucleatum, Lactobacillus rhamnosus, Neisseria subflava, Porphyromonas gingivalis, Actinomyces naeslundii, Prevotella nigrescens, Streptococcus oralis, Streptococcus sanguis, Streptococcus mutans and Veillonella dispar repeatedly to escalating, sublethal concentrations of triclosan. Escherichia coli ATCC 8739 was included as an organism showing the triclosan resistance development trait. MIC values towards chlorhexidine, metronidazole and tetracycline were determined before and after biocide exposure. Results: N. subflava, Pr. nigrescens Po. gingivalis and E. coli were highly susceptible to triclosan (MIC range 0.1-3.9 mg/L), whereas the lactobacillus and S. mutans were less susceptible (MIC range 15.6-20.8 mg/L). Triclosan exposure resulted in a highly significant (∼ 400-fold) reduction in triclosan susceptibility (P",

keywords = "Antibiotics, Dental plaque, Susceptibility",

author = "McBain, {Andrew J.} and Ledder, {Ruth G.} and Prem Sreenivasan and Peter Gilbert",

year = "2004",

month = may,

doi = "10.1093/jac/dkh168",

language = "English",

volume = "53",

pages = "772--777",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Selection for high-level resistance by chronic triclosan exposure is not universal

AU - McBain, Andrew J.

AU - Ledder, Ruth G.

AU - Sreenivasan, Prem

AU - Gilbert, Peter

PY - 2004/5

Y1 - 2004/5

N2 - Objectives: To investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria. Methods: A gradient plate technique was used to expose Fusobacterium nucleatum, Lactobacillus rhamnosus, Neisseria subflava, Porphyromonas gingivalis, Actinomyces naeslundii, Prevotella nigrescens, Streptococcus oralis, Streptococcus sanguis, Streptococcus mutans and Veillonella dispar repeatedly to escalating, sublethal concentrations of triclosan. Escherichia coli ATCC 8739 was included as an organism showing the triclosan resistance development trait. MIC values towards chlorhexidine, metronidazole and tetracycline were determined before and after biocide exposure. Results: N. subflava, Pr. nigrescens Po. gingivalis and E. coli were highly susceptible to triclosan (MIC range 0.1-3.9 mg/L), whereas the lactobacillus and S. mutans were less susceptible (MIC range 15.6-20.8 mg/L). Triclosan exposure resulted in a highly significant (∼ 400-fold) reduction in triclosan susceptibility (P

AB - Objectives: To investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria. Methods: A gradient plate technique was used to expose Fusobacterium nucleatum, Lactobacillus rhamnosus, Neisseria subflava, Porphyromonas gingivalis, Actinomyces naeslundii, Prevotella nigrescens, Streptococcus oralis, Streptococcus sanguis, Streptococcus mutans and Veillonella dispar repeatedly to escalating, sublethal concentrations of triclosan. Escherichia coli ATCC 8739 was included as an organism showing the triclosan resistance development trait. MIC values towards chlorhexidine, metronidazole and tetracycline were determined before and after biocide exposure. Results: N. subflava, Pr. nigrescens Po. gingivalis and E. coli were highly susceptible to triclosan (MIC range 0.1-3.9 mg/L), whereas the lactobacillus and S. mutans were less susceptible (MIC range 15.6-20.8 mg/L). Triclosan exposure resulted in a highly significant (∼ 400-fold) reduction in triclosan susceptibility (P

KW - Antibiotics

KW - Dental plaque

KW - Susceptibility

U2 - 10.1093/jac/dkh168

DO - 10.1093/jac/dkh168

M3 - Article

C2 - 15117935

SN - 0305-7453

VL - 53

SP - 772

EP - 777

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 5

ER -

Selection for high-level resistance by chronic triclosan exposure is not universal

Abstract

Keywords

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