Selection for high-level resistance by chronic triclosan exposure is not universal

Andrew J. McBain, Ruth G. Ledder, Prem Sreenivasan, Peter Gilbert

    Research output: Contribution to journalArticlepeer-review


    Objectives: To investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria. Methods: A gradient plate technique was used to expose Fusobacterium nucleatum, Lactobacillus rhamnosus, Neisseria subflava, Porphyromonas gingivalis, Actinomyces naeslundii, Prevotella nigrescens, Streptococcus oralis, Streptococcus sanguis, Streptococcus mutans and Veillonella dispar repeatedly to escalating, sublethal concentrations of triclosan. Escherichia coli ATCC 8739 was included as an organism showing the triclosan resistance development trait. MIC values towards chlorhexidine, metronidazole and tetracycline were determined before and after biocide exposure. Results: N. subflava, Pr. nigrescens Po. gingivalis and E. coli were highly susceptible to triclosan (MIC range 0.1-3.9 mg/L), whereas the lactobacillus and S. mutans were less susceptible (MIC range 15.6-20.8 mg/L). Triclosan exposure resulted in a highly significant (∼ 400-fold) reduction in triclosan susceptibility (P
    Original languageEnglish
    Pages (from-to)772-777
    Number of pages5
    JournalJournal of Antimicrobial Chemotherapy
    Issue number5
    Publication statusPublished - May 2004


    • Antibiotics
    • Dental plaque
    • Susceptibility


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