Selection of membrane protein targets for crystallization using PFO-PAGE electrophoresis

R. M. Cleverley, M. Saleem, J. Kean, R. C. Ford, J. P. Derrick, S. M. Prince

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A method to rapidly assess the oligomeric composition of multimeric proteins is notably absent from reported schemes for high throughput production and crystallization of membrane proteins. In this report we have investigated the suitability of PFO-PAGE electrophoresis for this purpose and present examples where it proves highly informative in selecting conditions favouring the functional oligomeric state of the target protein. Features such as the ability to analyze several samples in parallel, including crude membrane extracts, suggest it will be highly adaptable to high throughput analysis of membrane proteins. © 2008 Informa UK Ltd.
    Original languageEnglish
    Pages (from-to)625-630
    Number of pages5
    JournalMolecular Membrane Biology
    Volume25
    Issue number8
    DOIs
    Publication statusPublished - Dec 2008

    Keywords

    • ABC transporter
    • CorA
    • Membrane protein crystallization
    • Membrane protein purification
    • Pre-crystallization screening

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