Selective expression of mRNA coding for the truncated form of erythropoietin receptor in hematopoietic cells and its decrease in patients with polycythemia vera

Shigeru Chiba; Tokiharu Takahashi; Kenichi Takeshita; Jun Minowada; Yoshio Yazaki; Frank H. Ruddle; Hisamaru Hirai

Selective expression of mRNA coding for the truncated form of erythropoietin receptor in hematopoietic cells and its decrease in patients with polycythemia vera

Shigeru Chiba, Tokiharu Takahashi, Kenichi Takeshita, Jun Minowada, Yoshio Yazaki, Frank H. Ruddle, Hisamaru Hirai

Research output: Contribution to journal › Article › peer-review

Abstract

The mRNA encoding full-length erythropoietin (EPO) receptor (EPOR-F) comprises exons I through VIII. Another membrane-bound EPOR (EPOR-T) isoform has a truncated cytoplasmic region and is encoded by the mRNA containing unspliced intron VII (EPOR-T mRNA). EPOR-T is believed to have a dominantly negative function against EPOR-F. We show that EPOR-T mRNA is markedly decreased in the blood cells of patients with polycythemia vera (PV). We also show that EPOR-T mRNA is not detected in erythroid/megakaryocytic leukemia cell lines, but is expressed in nonerythroid/nonmegakaryocytic lines, suggesting the presence of a cell type-specific system by which intron VII of the EPOR transcript is spliced. Deregulation of this splicing system in early hematopoietic progenitors possibly explains the profound decrease in EPOR-T mRNA and consequent pathophysiology of PV.

Original language	English
Pages (from-to)	97-104
Number of pages	7
Journal	Blood
Volume	90
Issue number	1
Publication status	Published - 1 Jul 1997

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{2f6cf36654ce422ea35fe30b2901732a,

title = "Selective expression of mRNA coding for the truncated form of erythropoietin receptor in hematopoietic cells and its decrease in patients with polycythemia vera",

abstract = "The mRNA encoding full-length erythropoietin (EPO) receptor (EPOR-F) comprises exons I through VIII. Another membrane-bound EPOR (EPOR-T) isoform has a truncated cytoplasmic region and is encoded by the mRNA containing unspliced intron VII (EPOR-T mRNA). EPOR-T is believed to have a dominantly negative function against EPOR-F. We show that EPOR-T mRNA is markedly decreased in the blood cells of patients with polycythemia vera (PV). We also show that EPOR-T mRNA is not detected in erythroid/megakaryocytic leukemia cell lines, but is expressed in nonerythroid/nonmegakaryocytic lines, suggesting the presence of a cell type-specific system by which intron VII of the EPOR transcript is spliced. Deregulation of this splicing system in early hematopoietic progenitors possibly explains the profound decrease in EPOR-T mRNA and consequent pathophysiology of PV.",

author = "Shigeru Chiba and Tokiharu Takahashi and Kenichi Takeshita and Jun Minowada and Yoshio Yazaki and Ruddle, {Frank H.} and Hisamaru Hirai",

year = "1997",

month = jul,

day = "1",

language = "English",

volume = "90",

pages = "97--104",

journal = "Blood",

issn = "0006-4971",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - Selective expression of mRNA coding for the truncated form of erythropoietin receptor in hematopoietic cells and its decrease in patients with polycythemia vera

AU - Chiba, Shigeru

AU - Takahashi, Tokiharu

AU - Takeshita, Kenichi

AU - Minowada, Jun

AU - Yazaki, Yoshio

AU - Ruddle, Frank H.

AU - Hirai, Hisamaru

PY - 1997/7/1

Y1 - 1997/7/1

N2 - The mRNA encoding full-length erythropoietin (EPO) receptor (EPOR-F) comprises exons I through VIII. Another membrane-bound EPOR (EPOR-T) isoform has a truncated cytoplasmic region and is encoded by the mRNA containing unspliced intron VII (EPOR-T mRNA). EPOR-T is believed to have a dominantly negative function against EPOR-F. We show that EPOR-T mRNA is markedly decreased in the blood cells of patients with polycythemia vera (PV). We also show that EPOR-T mRNA is not detected in erythroid/megakaryocytic leukemia cell lines, but is expressed in nonerythroid/nonmegakaryocytic lines, suggesting the presence of a cell type-specific system by which intron VII of the EPOR transcript is spliced. Deregulation of this splicing system in early hematopoietic progenitors possibly explains the profound decrease in EPOR-T mRNA and consequent pathophysiology of PV.

AB - The mRNA encoding full-length erythropoietin (EPO) receptor (EPOR-F) comprises exons I through VIII. Another membrane-bound EPOR (EPOR-T) isoform has a truncated cytoplasmic region and is encoded by the mRNA containing unspliced intron VII (EPOR-T mRNA). EPOR-T is believed to have a dominantly negative function against EPOR-F. We show that EPOR-T mRNA is markedly decreased in the blood cells of patients with polycythemia vera (PV). We also show that EPOR-T mRNA is not detected in erythroid/megakaryocytic leukemia cell lines, but is expressed in nonerythroid/nonmegakaryocytic lines, suggesting the presence of a cell type-specific system by which intron VII of the EPOR transcript is spliced. Deregulation of this splicing system in early hematopoietic progenitors possibly explains the profound decrease in EPOR-T mRNA and consequent pathophysiology of PV.

M3 - Article

C2 - 9207443

SN - 0006-4971

VL - 90

SP - 97

EP - 104

JO - Blood

JF - Blood

IS - 1

ER -

Selective expression of mRNA coding for the truncated form of erythropoietin receptor in hematopoietic cells and its decrease in patients with polycythemia vera

Abstract

UN SDGs

Fingerprint

Cite this