Sensitive and universal method for microbial DNA extraction from blood products

N. Golbang, J. P. Burnie, P. E. Klapper, A. Rostock, P. Williamson

Research output: Contribution to journalArticlepeer-review

Abstract

A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This compared well with existing methodologies, worked on limited clinical samples and was not pathogen specific.

Original languageEnglish
Pages (from-to)861-863
Number of pages3
JournalJournal Of Clinical Pathology
Volume49
Issue number10
DOIs
Publication statusPublished - 1 Jan 1996

Keywords

  • DNA extraction
  • Microbes
  • PCR

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