Sensitive and universal method for microbial DNA extraction from blood products

N. Golbang; J. P. Burnie; P. E. Klapper; A. Rostock; P. Williamson

doi:10.1136/jcp.49.10.861

Sensitive and universal method for microbial DNA extraction from blood products

N. Golbang, J. P. Burnie^*, P. E. Klapper, A. Rostock, P. Williamson

^*Corresponding author for this work

Manchester Royal Infirmary Laboratory

Research output: Contribution to journal › Article › peer-review

Abstract

A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This compared well with existing methodologies, worked on limited clinical samples and was not pathogen specific.

Original language	English
Pages (from-to)	861-863
Number of pages	3
Journal	Journal Of Clinical Pathology
Volume	49
Issue number	10
DOIs	https://doi.org/10.1136/jcp.49.10.861
Publication status	Published - 1 Jan 1996

Keywords

DNA extraction
Microbes
PCR

Access to Document

10.1136/jcp.49.10.861

Cite this

@article{156f5db01b4d43ce942b1e917c77db24,

title = "Sensitive and universal method for microbial DNA extraction from blood products",

abstract = "A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This compared well with existing methodologies, worked on limited clinical samples and was not pathogen specific.",

keywords = "DNA extraction, Microbes, PCR",

author = "N. Golbang and Burnie, {J. P.} and Klapper, {P. E.} and A. Rostock and P. Williamson",

year = "1996",

month = jan,

day = "1",

doi = "10.1136/jcp.49.10.861",

language = "English",

volume = "49",

pages = "861--863",

journal = "Journal Of Clinical Pathology",

issn = "0021-9746",

publisher = "BMJ ",

number = "10",

}

TY - JOUR

T1 - Sensitive and universal method for microbial DNA extraction from blood products

AU - Golbang, N.

AU - Burnie, J. P.

AU - Klapper, P. E.

AU - Rostock, A.

AU - Williamson, P.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This compared well with existing methodologies, worked on limited clinical samples and was not pathogen specific.

AB - A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This compared well with existing methodologies, worked on limited clinical samples and was not pathogen specific.

KW - DNA extraction

KW - Microbes

KW - PCR

UR - http://www.scopus.com/inward/record.url?scp=0029797431&partnerID=8YFLogxK

U2 - 10.1136/jcp.49.10.861

DO - 10.1136/jcp.49.10.861

M3 - Article

C2 - 8943759

AN - SCOPUS:0029797431

SN - 0021-9746

VL - 49

SP - 861

EP - 863

JO - Journal Of Clinical Pathology

JF - Journal Of Clinical Pathology

IS - 10

ER -

Sensitive and universal method for microbial DNA extraction from blood products

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this