Separase Regulates INCENP-Aurora B Anaphase Spindle Function Through Cdc14

Gislene Pereira, Elmar Schiebel

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The inner centromere-like protein (INCENP) forms a complex with the evolutionarily conserved family of Aurora B kinases. The INCENP-Aurora complex helps coordinate chromosome segregation, spindle behavior, and cytokinesis during mitosis. INCENP-Aurora associates with kinetochores in metaphase and with spindle microtubules in anaphase, yet the trigger for this abrupt transfer is unknown. Here we show that the conserved phosphatase Cdc14 regulated the yeast INCENP-Aurora complex, Sli15-Ipl1. Cdc14 dephosphorylated Sli15 and thereby directed the complex to spindles. Activation of Cdc14 by separase was sufficient for Sli15 dephosphorylation and relocalization. Cdc14 not only regulates mitotic exit but also modulates spindle midzone assembly through Sli15-Ipl1.
    Original languageEnglish
    Pages (from-to)2120-2124
    Number of pages4
    JournalScience
    Volume302
    Issue number5653
    DOIs
    Publication statusPublished - 19 Dec 2003

    Fingerprint

    Dive into the research topics of 'Separase Regulates INCENP-Aurora B Anaphase Spindle Function Through Cdc14'. Together they form a unique fingerprint.

    Cite this