Separating Overlapping Tissue Layers from Microscopy Images

Research output: Working paperPreprint


Manual preparation of tissue slices for microscopy imaging can introduce tissue tears and overlaps. Typically, further digital processing algorithms such as registration and 3D reconstruction from tissue image stacks cannot handle images with tissue tear/overlap artifacts, and so such images are usually discarded. In this paper, we propose an imaging model and an algorithm to digitally separate overlapping tissue data of mouse brain images into two layers. We show the correctness of our model and the algorithm by comparing our results with the ground truth.
Original languageEnglish
Place of PublicationIthaca
Number of pages5
Publication statusPublished - 22 May 2019


  • Overlaid artifact
  • image separation
  • texture synthesis
  • Microscopy imaging model
  • overlapping images


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