Separating the inseparable: The metabolomic analysis of plant-pathogen interactions

J. William Allwood; Jim Heald; Amanda J. Lloyd; Royston Goodacre; Luis A J Mur

doi:10.1007/978-1-61779-594-7_3

Separating the inseparable: The metabolomic analysis of plant-pathogen interactions

J. William Allwood, Jim Heald, Amanda J. Lloyd, Royston Goodacre, Luis A J Mur

Research output: Contribution to journal › Article › peer-review

Abstract

Plant-microbe interactions-whether pathogenic or symbiotic-exert major influences on plant physiology and productivity. Analysis of such interactions represents a particular challenge to metabolomic approaches due to the intimate association between the interacting partners coupled with a general commonality of metabolites. We here describe an approach based on co-cultivation of Arabidopsis cell cultures and bacterial plant pathogens to assess the metabolomes of both interacting partners, which we refer to as dual metabolomics. © 2011 Springer Science+Business Media, LLC.

Original language	English
Pages (from-to)	31-49
Number of pages	18
Journal	Methods in Molecular Biology
Volume	860
DOIs	https://doi.org/10.1007/978-1-61779-594-7_3
Publication status	Published - 2012

Keywords

Arabidopsis
Co-cultivation
Dual metabolomics
Pathogen
Plant suspension cultures
Plant-microbe interactions

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10.1007/978-1-61779-594-7_3

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abstract = "Plant-microbe interactions-whether pathogenic or symbiotic-exert major influences on plant physiology and productivity. Analysis of such interactions represents a particular challenge to metabolomic approaches due to the intimate association between the interacting partners coupled with a general commonality of metabolites. We here describe an approach based on co-cultivation of Arabidopsis cell cultures and bacterial plant pathogens to assess the metabolomes of both interacting partners, which we refer to as dual metabolomics. {\textcopyright} 2011 Springer Science+Business Media, LLC.",

keywords = "Arabidopsis, Co-cultivation, Dual metabolomics, Pathogen, Plant suspension cultures, Plant-microbe interactions",

author = "Allwood, {J. William} and Jim Heald and Lloyd, {Amanda J.} and Royston Goodacre and Mur, {Luis A J}",

note = ", Biotechnology and Biological Sciences Research Council, United Kingdom",

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journal = "Methods in Molecular Biology",

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T1 - Separating the inseparable: The metabolomic analysis of plant-pathogen interactions

AU - Allwood, J. William

AU - Heald, Jim

AU - Lloyd, Amanda J.

AU - Goodacre, Royston

AU - Mur, Luis A J

N1 - , Biotechnology and Biological Sciences Research Council, United Kingdom

PY - 2012

Y1 - 2012

N2 - Plant-microbe interactions-whether pathogenic or symbiotic-exert major influences on plant physiology and productivity. Analysis of such interactions represents a particular challenge to metabolomic approaches due to the intimate association between the interacting partners coupled with a general commonality of metabolites. We here describe an approach based on co-cultivation of Arabidopsis cell cultures and bacterial plant pathogens to assess the metabolomes of both interacting partners, which we refer to as dual metabolomics. © 2011 Springer Science+Business Media, LLC.

AB - Plant-microbe interactions-whether pathogenic or symbiotic-exert major influences on plant physiology and productivity. Analysis of such interactions represents a particular challenge to metabolomic approaches due to the intimate association between the interacting partners coupled with a general commonality of metabolites. We here describe an approach based on co-cultivation of Arabidopsis cell cultures and bacterial plant pathogens to assess the metabolomes of both interacting partners, which we refer to as dual metabolomics. © 2011 Springer Science+Business Media, LLC.

KW - Arabidopsis

KW - Co-cultivation

KW - Dual metabolomics

KW - Pathogen

KW - Plant suspension cultures

KW - Plant-microbe interactions

U2 - 10.1007/978-1-61779-594-7_3

DO - 10.1007/978-1-61779-594-7_3

M3 - Article

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SN - 1064-3745

VL - 860

SP - 31

EP - 49

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Separating the inseparable: The metabolomic analysis of plant-pathogen interactions

Abstract

Keywords

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