Separation of glucose oxidase and catalase using ultrafiltration with 300-kDa polyethersulfone membranes

Jianguo Liu; Junren Lu; Xiubo Zhao; Jianren Lu; Zhanfeng Cui

doi:10.1016/j.memsci.2007.04.044

Separation of glucose oxidase and catalase using ultrafiltration with 300-kDa polyethersulfone membranes

Jianguo Liu, Junren Lu, Xiubo Zhao, Jianren Lu, Zhanfeng Cui

Research output: Contribution to journal › Article › peer-review

Abstract

This work examines the separation of glucose oxidase (GOx) and catalase by ultrafiltration using 300 kDa MWCO polyethersulfone membranes. The effects of solution pH and ionic strength on the transmission of a single protein (either GOx or catalase) were quantified using the pulsed sample injection technique. Other effects including stirring speed and permeate flux on separation of the binary protein mixtures were also studied using parameter scanning ultrafiltration. Under optimized conditions, glucose oxidase transmission could reach more than 47% and the purity obtained in the permeate was greater than 93% after one stage ultrafiltration process even with higher molar ratio of catalase in the feed. © 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	222-228
Number of pages	6
Journal	Journal of Membrane Science
Volume	299
Issue number	1-2
DOIs	https://doi.org/10.1016/j.memsci.2007.04.044
Publication status	Published - 1 Aug 2007

Keywords

Catalase
Glucose oxidase
Membrane
Protein fractionation
Ultrafiltration

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10.1016/j.memsci.2007.04.044

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title = "Separation of glucose oxidase and catalase using ultrafiltration with 300-kDa polyethersulfone membranes",

abstract = "This work examines the separation of glucose oxidase (GOx) and catalase by ultrafiltration using 300 kDa MWCO polyethersulfone membranes. The effects of solution pH and ionic strength on the transmission of a single protein (either GOx or catalase) were quantified using the pulsed sample injection technique. Other effects including stirring speed and permeate flux on separation of the binary protein mixtures were also studied using parameter scanning ultrafiltration. Under optimized conditions, glucose oxidase transmission could reach more than 47% and the purity obtained in the permeate was greater than 93% after one stage ultrafiltration process even with higher molar ratio of catalase in the feed. {\textcopyright} 2007 Elsevier B.V. All rights reserved.",

keywords = "Catalase, Glucose oxidase, Membrane, Protein fractionation, Ultrafiltration",

author = "Jianguo Liu and Junren Lu and Xiubo Zhao and Jianren Lu and Zhanfeng Cui",

year = "2007",

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doi = "10.1016/j.memsci.2007.04.044",

language = "English",

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TY - JOUR

T1 - Separation of glucose oxidase and catalase using ultrafiltration with 300-kDa polyethersulfone membranes

AU - Liu, Jianguo

AU - Lu, Junren

AU - Zhao, Xiubo

AU - Lu, Jianren

AU - Cui, Zhanfeng

PY - 2007/8/1

Y1 - 2007/8/1

N2 - This work examines the separation of glucose oxidase (GOx) and catalase by ultrafiltration using 300 kDa MWCO polyethersulfone membranes. The effects of solution pH and ionic strength on the transmission of a single protein (either GOx or catalase) were quantified using the pulsed sample injection technique. Other effects including stirring speed and permeate flux on separation of the binary protein mixtures were also studied using parameter scanning ultrafiltration. Under optimized conditions, glucose oxidase transmission could reach more than 47% and the purity obtained in the permeate was greater than 93% after one stage ultrafiltration process even with higher molar ratio of catalase in the feed. © 2007 Elsevier B.V. All rights reserved.

AB - This work examines the separation of glucose oxidase (GOx) and catalase by ultrafiltration using 300 kDa MWCO polyethersulfone membranes. The effects of solution pH and ionic strength on the transmission of a single protein (either GOx or catalase) were quantified using the pulsed sample injection technique. Other effects including stirring speed and permeate flux on separation of the binary protein mixtures were also studied using parameter scanning ultrafiltration. Under optimized conditions, glucose oxidase transmission could reach more than 47% and the purity obtained in the permeate was greater than 93% after one stage ultrafiltration process even with higher molar ratio of catalase in the feed. © 2007 Elsevier B.V. All rights reserved.

KW - Catalase

KW - Glucose oxidase

KW - Membrane

KW - Protein fractionation

KW - Ultrafiltration

U2 - 10.1016/j.memsci.2007.04.044

DO - 10.1016/j.memsci.2007.04.044

M3 - Article

SN - 1873-3123

VL - 299

SP - 222

EP - 228

JO - Journal of Membrane Science

JF - Journal of Membrane Science

IS - 1-2

ER -

Separation of glucose oxidase and catalase using ultrafiltration with 300-kDa polyethersulfone membranes

Abstract

Keywords

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