Sequential development of hematopoietic and cardiac mesoderm during embryonic stem cell differentiation

Valerie Kouskoff, Georges Lacaud, Staci Schwantz, Hans Jöerg Fehling, Gordon Keller

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The ability to generate a wide spectrum of differentiated cell types from ES cells in culture offers a powerful approach for studying lineage induction and specification and a promising source of progenitors for cell replacement therapy. Although significant efforts are being made to optimize culture conditions for the generation of different cell populations from ES cells, the identification and efficient isolation of specific progenitors for many lineages within these cultures remains a major challenge. By specifically tracking hematopoietic and cardiac development, we demonstrate here that these two lineages arise from distinct mesoderm subpopulations that develop in sequential waves from pre-mesoderm cells. Access to these populations provides a unique approach to isolate and characterize the earliest progenitors of these lineages. © 2005 by The National Academy of Sciences of the USA.
    Original languageEnglish
    Pages (from-to)13170-13175
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume102
    Issue number37
    DOIs
    Publication statusPublished - 13 Sep 2005

    Keywords

    • Brachyury
    • Cardiogenesis
    • Hematopoiesis

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