Serological diagnosis of varicella-zoster virus in sera with antibody-capture enzyme-linked immunosorbent assay of IgM

D. K. Oladepo; P. E. Klapper; D. Percival; P. J. Vallely

doi:10.1016/S0166-0934(99)00139-1

Serological diagnosis of varicella-zoster virus in sera with antibody-capture enzyme-linked immunosorbent assay of IgM

D. K. Oladepo^*
, P. E. Klapper
, D. Percival
, P. J. Vallely

^*Corresponding author for this work

Division of Immunology, Immunity to Infection and Respiratory Medicine

Cortecs Diagnostics

Research output: Contribution to journal › Article › peer-review

Abstract

Evaluation was made of three enzyme-linked immunosorbent assay (ELISA) formats; varicella-zoster virus (VZV) indirect ELISA; VZV IgM capture using biotin and VZV IgM capture using peroxidase, for the detection of VZV-specific IgM antibodies in human sera. It was observed that there was no significant difference in sensitivity of detection using the three formats but there were important practical differences in the number of steps and hence time for assay completion between the three assay formats. All assays showed some cross-reactivity with sera containing anti-HSV1 antibodies.

Original language	English
Pages (from-to)	169-173
Number of pages	5
Journal	Journal of Virological Methods
Volume	84
Issue number	2
DOIs	https://doi.org/10.1016/S0166-0934(99)00139-1
Publication status	Published - Feb 2000

Keywords

Biotin
ELISA
IgM
Indirect
Peroxidase
VZV

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10.1016/S0166-0934(99)00139-1

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title = "Serological diagnosis of varicella-zoster virus in sera with antibody-capture enzyme-linked immunosorbent assay of IgM",

abstract = "Evaluation was made of three enzyme-linked immunosorbent assay (ELISA) formats; varicella-zoster virus (VZV) indirect ELISA; VZV IgM capture using biotin and VZV IgM capture using peroxidase, for the detection of VZV-specific IgM antibodies in human sera. It was observed that there was no significant difference in sensitivity of detection using the three formats but there were important practical differences in the number of steps and hence time for assay completion between the three assay formats. All assays showed some cross-reactivity with sera containing anti-HSV1 antibodies.",

keywords = "Biotin, ELISA, IgM, Indirect, Peroxidase, VZV",

author = "Oladepo, \{D. K.\} and Klapper, \{P. E.\} and D. Percival and Vallely, \{P. J.\}",

year = "2000",

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doi = "10.1016/S0166-0934(99)00139-1",

language = "English",

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T1 - Serological diagnosis of varicella-zoster virus in sera with antibody-capture enzyme-linked immunosorbent assay of IgM

AU - Oladepo, D. K.

AU - Klapper, P. E.

AU - Percival, D.

AU - Vallely, P. J.

PY - 2000/2

Y1 - 2000/2

N2 - Evaluation was made of three enzyme-linked immunosorbent assay (ELISA) formats; varicella-zoster virus (VZV) indirect ELISA; VZV IgM capture using biotin and VZV IgM capture using peroxidase, for the detection of VZV-specific IgM antibodies in human sera. It was observed that there was no significant difference in sensitivity of detection using the three formats but there were important practical differences in the number of steps and hence time for assay completion between the three assay formats. All assays showed some cross-reactivity with sera containing anti-HSV1 antibodies.

AB - Evaluation was made of three enzyme-linked immunosorbent assay (ELISA) formats; varicella-zoster virus (VZV) indirect ELISA; VZV IgM capture using biotin and VZV IgM capture using peroxidase, for the detection of VZV-specific IgM antibodies in human sera. It was observed that there was no significant difference in sensitivity of detection using the three formats but there were important practical differences in the number of steps and hence time for assay completion between the three assay formats. All assays showed some cross-reactivity with sera containing anti-HSV1 antibodies.

KW - Biotin

KW - ELISA

KW - IgM

KW - Indirect

KW - Peroxidase

KW - VZV

UR - https://www.scopus.com/pages/publications/0033984687#tab=citedBy

UR - https://www.scopus.com/pages/publications/0033984687

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DO - 10.1016/S0166-0934(99)00139-1

M3 - Article

C2 - 10680966

AN - SCOPUS:0033984687

SN - 0166-0934

VL - 84

SP - 169

EP - 173

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

Serological diagnosis of varicella-zoster virus in sera with antibody-capture enzyme-linked immunosorbent assay of IgM

Abstract

Keywords

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