SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal

Olivier Binda; Ana Sevilla; Gary LeRoy; Ihor R. Lemischka; Benjamin A. Garcia; Stéphane Richard

doi:10.4161/epi.23416

SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal

Olivier Binda, Ana Sevilla, Gary LeRoy, Ihor R. Lemischka, Benjamin A. Garcia, Stéphane Richard

Research output: Contribution to journal › Article › peer-review

Abstract

The histone H2A variant H2AZ is an essential chromatin signaling factor. Herein, we report that H2AZ is monomethylated at lysine 7 (H2AZK7me1) by the lysine methyltransferase SETD6. We observed that methylation of H2AZ increased noticeably upon cellular differentiation of mouse embryonic stem cells (mESC s). H2AZK7me1 and the repressive H3K27me3 mark were found near the transcriptional start sites of differentiation marker genes, but were removed upon retinoic acid-induced cellular differentiation. The depletion of Setd6 in mESC s led to cellular differentiation, compromised self-renewal, and poor clonogenicity. These findings demonstrate that mESC s require Setd6 for self-renewal and portray H2AZK7me1 as a marker of cellular differentiation. © 2013 Landes Bioscience.

Original language	English
Pages (from-to)	177-183
Number of pages	6
Journal	Epigenetics: official journal of the DNA Methylation Society
Volume	8
Issue number	2
DOIs	https://doi.org/10.4161/epi.23416
Publication status	Published - 2013

Keywords

Embryonic stem cells
H2AZ
Histone variant
Lysine methylation
Lysine methyltransferase
SETD6

Access to Document

10.4161/epi.23416

http://www.landesbioscience.com/journals/epigenetics/2012EPI0355.pdf?nocache=1131157004

Cite this

@article{d187eabd77924b2887a7b7d594305f6e,

title = "SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal",

abstract = "The histone H2A variant H2AZ is an essential chromatin signaling factor. Herein, we report that H2AZ is monomethylated at lysine 7 (H2AZK7me1) by the lysine methyltransferase SETD6. We observed that methylation of H2AZ increased noticeably upon cellular differentiation of mouse embryonic stem cells (mESC s). H2AZK7me1 and the repressive H3K27me3 mark were found near the transcriptional start sites of differentiation marker genes, but were removed upon retinoic acid-induced cellular differentiation. The depletion of Setd6 in mESC s led to cellular differentiation, compromised self-renewal, and poor clonogenicity. These findings demonstrate that mESC s require Setd6 for self-renewal and portray H2AZK7me1 as a marker of cellular differentiation. {\textcopyright} 2013 Landes Bioscience.",

keywords = "Embryonic stem cells, H2AZ, Histone variant, Lysine methylation, Lysine methyltransferase, SETD6",

author = "Olivier Binda and Ana Sevilla and Gary LeRoy and Lemischka, {Ihor R.} and Garcia, {Benjamin A.} and St{\'e}phane Richard",

note = "5R01GM078465, NIGMS NIH HHS, United StatesDP2OD007447, NIH HHS, United StatesMOP-67070, Canadian Institutes of Health Research, CanadaMOP-93811, Canadian Institutes of Health Research, Canada, Wellcome Trust, United Kingdom",

year = "2013",

doi = "10.4161/epi.23416",

language = "English",

volume = "8",

pages = "177--183",

journal = "Epigenetics: official journal of the DNA Methylation Society",

issn = "1559-2308",

publisher = "Landes Bioscience",

number = "2",

}

TY - JOUR

T1 - SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal

AU - Binda, Olivier

AU - Sevilla, Ana

AU - LeRoy, Gary

AU - Lemischka, Ihor R.

AU - Garcia, Benjamin A.

AU - Richard, Stéphane

N1 - 5R01GM078465, NIGMS NIH HHS, United StatesDP2OD007447, NIH HHS, United StatesMOP-67070, Canadian Institutes of Health Research, CanadaMOP-93811, Canadian Institutes of Health Research, Canada, Wellcome Trust, United Kingdom

PY - 2013

Y1 - 2013

N2 - The histone H2A variant H2AZ is an essential chromatin signaling factor. Herein, we report that H2AZ is monomethylated at lysine 7 (H2AZK7me1) by the lysine methyltransferase SETD6. We observed that methylation of H2AZ increased noticeably upon cellular differentiation of mouse embryonic stem cells (mESC s). H2AZK7me1 and the repressive H3K27me3 mark were found near the transcriptional start sites of differentiation marker genes, but were removed upon retinoic acid-induced cellular differentiation. The depletion of Setd6 in mESC s led to cellular differentiation, compromised self-renewal, and poor clonogenicity. These findings demonstrate that mESC s require Setd6 for self-renewal and portray H2AZK7me1 as a marker of cellular differentiation. © 2013 Landes Bioscience.

AB - The histone H2A variant H2AZ is an essential chromatin signaling factor. Herein, we report that H2AZ is monomethylated at lysine 7 (H2AZK7me1) by the lysine methyltransferase SETD6. We observed that methylation of H2AZ increased noticeably upon cellular differentiation of mouse embryonic stem cells (mESC s). H2AZK7me1 and the repressive H3K27me3 mark were found near the transcriptional start sites of differentiation marker genes, but were removed upon retinoic acid-induced cellular differentiation. The depletion of Setd6 in mESC s led to cellular differentiation, compromised self-renewal, and poor clonogenicity. These findings demonstrate that mESC s require Setd6 for self-renewal and portray H2AZK7me1 as a marker of cellular differentiation. © 2013 Landes Bioscience.

KW - Embryonic stem cells

KW - H2AZ

KW - Histone variant

KW - Lysine methylation

KW - Lysine methyltransferase

KW - SETD6

U2 - 10.4161/epi.23416

DO - 10.4161/epi.23416

M3 - Article

C2 - 23324626

SN - 1559-2308

VL - 8

SP - 177

EP - 183

JO - Epigenetics: official journal of the DNA Methylation Society

JF - Epigenetics: official journal of the DNA Methylation Society

IS - 2

ER -

SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal

Abstract

Keywords

Access to Document

Fingerprint

Cite this