Sex differences in SR Ca(2+) release in murine ventricular myocytes are regulated by the cAMP/PKA pathway.

Randi J Parks; Gibanananda Ray; Laura A Bienvenu; Robert A Rose; Susan E Howlett

doi:10.1016/j.yjmcc.2014.07.006

Sex differences in SR Ca(2+) release in murine ventricular myocytes are regulated by the cAMP/PKA pathway.

Randi J Parks, Gibanananda Ray, Laura A Bienvenu, Robert A Rose, Susan E Howlett

Research output: Contribution to journal › Article › peer-review

Abstract

Previous studies have shown that ventricular myocytes from female rats have smaller contractions and Ca(2+) transients than males. As cardiac contraction is regulated by the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway, we hypothesized that sex differences in cAMP contribute to differences in Ca(2+) handling. Ca(2+) transients (fura-2) and ionic currents were measured simultaneously (37°C, 2Hz) in ventricular myocytes from adult male and female C57BL/6 mice. Under basal conditions, diastolic Ca(2+), sarcoplasmic reticulum (SR) Ca(2+) stores, and L-type Ca(2+) current did not differ between the sexes. However, female myocytes had smaller Ca(2+) transients (26% smaller), Ca(2+) sparks (6% smaller), and excitation-contraction coupling gain in comparison to males (23% smaller). Interestingly, basal levels of intracellular cAMP were lower in female myocytes (0.7±0.1 vs. 1.7±0.2fmol/μg protein; p

Original language	English
Journal	Journal of molecular and cellular cardiology
Volume	75
DOIs	https://doi.org/10.1016/j.yjmcc.2014.07.006
Publication status	Published - Oct 2014

Keywords

Calcium spark
Calcium transient
Excitation–contraction coupling
Gain
Gender
Phosphodiesterase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.yjmcc.2014.07.006

Cite this

@article{6ad9f68ee94a4e2a815564007c3d5281,

title = "Sex differences in SR Ca(2+) release in murine ventricular myocytes are regulated by the cAMP/PKA pathway.",

abstract = "Previous studies have shown that ventricular myocytes from female rats have smaller contractions and Ca(2+) transients than males. As cardiac contraction is regulated by the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway, we hypothesized that sex differences in cAMP contribute to differences in Ca(2+) handling. Ca(2+) transients (fura-2) and ionic currents were measured simultaneously (37°C, 2Hz) in ventricular myocytes from adult male and female C57BL/6 mice. Under basal conditions, diastolic Ca(2+), sarcoplasmic reticulum (SR) Ca(2+) stores, and L-type Ca(2+) current did not differ between the sexes. However, female myocytes had smaller Ca(2+) transients (26% smaller), Ca(2+) sparks (6% smaller), and excitation-contraction coupling gain in comparison to males (23% smaller). Interestingly, basal levels of intracellular cAMP were lower in female myocytes (0.7±0.1 vs. 1.7±0.2fmol/μg protein; p",

keywords = "Calcium spark, Calcium transient, Excitation–contraction coupling, Gain, Gender, Phosphodiesterase",

author = "Parks, {Randi J} and Gibanananda Ray and Bienvenu, {Laura A} and Rose, {Robert A} and Howlett, {Susan E}",

note = "MOP97973, Canadian Institutes of Health Research, Canada",

year = "2014",

month = oct,

doi = "10.1016/j.yjmcc.2014.07.006",

language = "English",

volume = "75",

journal = "Journal of molecular and cellular cardiology",

issn = "1095-8584",

publisher = "Academic Press",

}

TY - JOUR

T1 - Sex differences in SR Ca(2+) release in murine ventricular myocytes are regulated by the cAMP/PKA pathway.

AU - Parks, Randi J

AU - Ray, Gibanananda

AU - Bienvenu, Laura A

AU - Rose, Robert A

AU - Howlett, Susan E

N1 - MOP97973, Canadian Institutes of Health Research, Canada

PY - 2014/10

Y1 - 2014/10

N2 - Previous studies have shown that ventricular myocytes from female rats have smaller contractions and Ca(2+) transients than males. As cardiac contraction is regulated by the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway, we hypothesized that sex differences in cAMP contribute to differences in Ca(2+) handling. Ca(2+) transients (fura-2) and ionic currents were measured simultaneously (37°C, 2Hz) in ventricular myocytes from adult male and female C57BL/6 mice. Under basal conditions, diastolic Ca(2+), sarcoplasmic reticulum (SR) Ca(2+) stores, and L-type Ca(2+) current did not differ between the sexes. However, female myocytes had smaller Ca(2+) transients (26% smaller), Ca(2+) sparks (6% smaller), and excitation-contraction coupling gain in comparison to males (23% smaller). Interestingly, basal levels of intracellular cAMP were lower in female myocytes (0.7±0.1 vs. 1.7±0.2fmol/μg protein; p

AB - Previous studies have shown that ventricular myocytes from female rats have smaller contractions and Ca(2+) transients than males. As cardiac contraction is regulated by the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway, we hypothesized that sex differences in cAMP contribute to differences in Ca(2+) handling. Ca(2+) transients (fura-2) and ionic currents were measured simultaneously (37°C, 2Hz) in ventricular myocytes from adult male and female C57BL/6 mice. Under basal conditions, diastolic Ca(2+), sarcoplasmic reticulum (SR) Ca(2+) stores, and L-type Ca(2+) current did not differ between the sexes. However, female myocytes had smaller Ca(2+) transients (26% smaller), Ca(2+) sparks (6% smaller), and excitation-contraction coupling gain in comparison to males (23% smaller). Interestingly, basal levels of intracellular cAMP were lower in female myocytes (0.7±0.1 vs. 1.7±0.2fmol/μg protein; p

KW - Calcium spark

KW - Calcium transient

KW - Excitation–contraction coupling

KW - Gain

KW - Gender

KW - Phosphodiesterase

U2 - 10.1016/j.yjmcc.2014.07.006

DO - 10.1016/j.yjmcc.2014.07.006

M3 - Article

C2 - 25066697

SN - 1095-8584

VL - 75

JO - Journal of molecular and cellular cardiology

JF - Journal of molecular and cellular cardiology

ER -

Sex differences in SR Ca(2+) release in murine ventricular myocytes are regulated by the cAMP/PKA pathway.

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this