TY - JOUR
T1 - Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor-like subunit with homology to the Drosophila melanogaster Dβ1 subunit
AU - Jones, A. K.
AU - Marshall, J.
AU - Blake, A. D.
AU - Buckingham, S. D.
AU - Darlison, M. G.
AU - Sattelle, D. B.
PY - 2005/11
Y1 - 2005/11
N2 - The cloning, sequencing and functional expression of Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor (nAChR) subunit is described. This subunit shows 80% identity with the Drosophila melanogaster Dβ1 and 92% identity with the Locusta migratoria β1, non-α subunits but only 38% identity to Sgα1 (also referred to as αL1), a previously cloned S. gregaria nAChR α-subunit. When expressed in Xenopus laevis oocytes, Sgβ1 does not respond to nicotine. Responses to nicotine are observed, however, in oocytes co-expressing Sgα1 and Sgβ1, but the pharmacology is indistinguishable from that of currents produced by expressing Sgα1 alone. We conclude that either Sgβ1 does not co-assemble with Sgα1, or that it is unable to contribute to the functional properties of the receptor, in the Xenopus oocyte expression system. © Springer-Verlag 2005.
AB - The cloning, sequencing and functional expression of Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor (nAChR) subunit is described. This subunit shows 80% identity with the Drosophila melanogaster Dβ1 and 92% identity with the Locusta migratoria β1, non-α subunits but only 38% identity to Sgα1 (also referred to as αL1), a previously cloned S. gregaria nAChR α-subunit. When expressed in Xenopus laevis oocytes, Sgβ1 does not respond to nicotine. Responses to nicotine are observed, however, in oocytes co-expressing Sgα1 and Sgβ1, but the pharmacology is indistinguishable from that of currents produced by expressing Sgα1 alone. We conclude that either Sgβ1 does not co-assemble with Sgα1, or that it is unable to contribute to the functional properties of the receptor, in the Xenopus oocyte expression system. © Springer-Verlag 2005.
KW - Locust (Schistocerca gregaria)
KW - Nicotinic acetylcholine receptor
KW - Nicotinic receptor ligands
KW - Sgβ1 non-α subunit
KW - Xenopus oocyte expression
U2 - 10.1007/s10158-005-0007-6
DO - 10.1007/s10158-005-0007-6
M3 - Article
C2 - 16177887
SN - 1354-2516
VL - 5
SP - 147
EP - 155
JO - Invertebrate Neuroscience
JF - Invertebrate Neuroscience
IS - 3-4
ER -