TY - CHAP
T1 - Shining light on the microbial world the application of Raman microspectroscopy.
AU - Huang, Wei E.
AU - Li, Mengqiu
AU - Jarvis, Roger M.
AU - Goodacre, Royston
AU - Banwart, Steven A.
N1 - Times Cited: 6
PY - 2010
Y1 - 2010
N2 - Raman microspectroscopy is a noninvasive, label-free, and single-cell technology for biochemical analysis of individual mammalian cells, organelles, bacteria, viruses, and nanoparticles. Chemical information derived from a Raman spectrum provides comprehensive and intrinsic information (e.g., nucleic acids, protein, carbohydrates, and lipids) of single cells without the need of any external labeling. A Raman spectrum functions as a molecular "fingerprint" of single cells, which enables the differentiation of cell types, physiological states, nutrient condition, and variable phenotypes. Raman microspectroscopy combined with stable isotope probing, fluorescent in situ hybridization, and optical tweezers offers a culture-independent approach to study the functions and physiology of unculturable microorganisms in the ecosystem. Here, we review the application of Raman microspectroscopy to microbiology research with particular emphasis on single bacterial cells. Copyright 2010 Elsevier Inc. All rights reserved.
AB - Raman microspectroscopy is a noninvasive, label-free, and single-cell technology for biochemical analysis of individual mammalian cells, organelles, bacteria, viruses, and nanoparticles. Chemical information derived from a Raman spectrum provides comprehensive and intrinsic information (e.g., nucleic acids, protein, carbohydrates, and lipids) of single cells without the need of any external labeling. A Raman spectrum functions as a molecular "fingerprint" of single cells, which enables the differentiation of cell types, physiological states, nutrient condition, and variable phenotypes. Raman microspectroscopy combined with stable isotope probing, fluorescent in situ hybridization, and optical tweezers offers a culture-independent approach to study the functions and physiology of unculturable microorganisms in the ecosystem. Here, we review the application of Raman microspectroscopy to microbiology research with particular emphasis on single bacterial cells. Copyright 2010 Elsevier Inc. All rights reserved.
U2 - 10.1016/S0065-2164(10)70005-8
DO - 10.1016/S0065-2164(10)70005-8
M3 - Chapter
VL - 70
T3 - Advances in Applied Microbiology
SP - 153
EP - 186
BT - Advances in applied microbiology|Adv. Appl. Microbiol.
ER -