Silencing miR-370-3p rescues funny current and sinus node function in heart failure

Joseph Yanni Gerges, Alicia D'Souza, Yanwen Wang, Ning Li, Brian J Hansen, Stanislav Zakharkin, Matthew Smith, Christina Hayward, Bryan A Whitson, Peter J. Mohler, Paul M L Janssen, Leo Zeef, Moinuddin Choudhury, Min Zi, Xue Cai, Sunil Logantha, Shu Nakao, Andrew Atkinson, Maria Petkova, Ursula DorisJonathan Ariyaratnam, Elizabeth Cartwright, Sam Griffiths-Jones, George Hart, Vadim V. Federov, Delvac Oceandy, Halina Dobrzynski, Mark Boyett

Research output: Contribution to journalArticlepeer-review


Bradyarrhythmias are an important cause of mortality in heart failure and previous studies indicate a mechanistic role for electrical remodelling of the key pacemaking ion channel HCN4 in this process. Here we show that, in a mouse model of heart failure in which there is sinus bradycardia, there is upregulation of a microRNA (miR-370-3p), downregulation of the pacemaker ion channel, HCN4, and downregulation of the corresponding ionic current, If, in the sinus node. In vitro, exogenous miR-370-3p inhibits HCN4 mRNA and causes downregulation of HCN4 protein, downregulation of If , and bradycardia in the isolated sinus node. In vivo, intraperitoneal injection of an antimiR to miR-370-3p into heart failure mice silences miR-370-3p and restores HCN4 mRNA and protein and If in the sinus node and blunts the sinus bradycardia. In addition, it partially restores ventricular function and reduces mortality. This represents a novel approach to heart failure treatment.
Original languageEnglish
Article number11279
JournalScientific Reports
Publication statusPublished - 9 Jul 2020


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