Simple cytokeratins in the serum of patients with lung cancer: Relationship to cell death

N. Pendleton; N. L. Occleston; M. J. Walshaw; M. W. Myskow; J. A H Littler; J. A. Green

Simple cytokeratins in the serum of patients with lung cancer: Relationship to cell death

N. Pendleton, N. L. Occleston, M. J. Walshaw, M. W. Myskow, J. A H Littler, J. A. Green

Research output: Contribution to journal › Article › peer-review

Abstract

An important role in differentiation and proliferation has been demonstrated for the 20 cytokeratin (CK) polypeptides. The serum of 24 patients with biopsy-proven non-small cell lung cancer (NSCLC) and a similar number of controls was examined for evidence of CK8 and CK18. Using enzyme-linked immunosorbent assay (ELISA), all the control sera were negative, but 9 of the 24 patients were positive (mean 2.62 ng/ml; range 1.4-5.8; P = 0.0036). Western blotting confirmed the results of the ELISA in all cases, and indicated full size CK polypeptides. Advanced stage disease patients were more likely to be seropositive (P = 0.00024). Biopsy specimens showed CK8 expression in all 24 cases by immunochemistry and CK18 in 22 cases. This is the first study to demonstrate that a subgroup of NSCLC patients have intact CK8 and CK18 peptides in their serum, and their detection may correlate with advanced disease. © 1994 Elsevier Science Ltd.

Original language	English
Pages (from-to)	93-96
Number of pages	3
Journal	European Journal of Cancer
Volume	30
Issue number	1
Publication status	Published - 1994

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@article{11af431f1dba4a70be3bf0bae04d4e3f,

title = "Simple cytokeratins in the serum of patients with lung cancer: Relationship to cell death",

abstract = "An important role in differentiation and proliferation has been demonstrated for the 20 cytokeratin (CK) polypeptides. The serum of 24 patients with biopsy-proven non-small cell lung cancer (NSCLC) and a similar number of controls was examined for evidence of CK8 and CK18. Using enzyme-linked immunosorbent assay (ELISA), all the control sera were negative, but 9 of the 24 patients were positive (mean 2.62 ng/ml; range 1.4-5.8; P = 0.0036). Western blotting confirmed the results of the ELISA in all cases, and indicated full size CK polypeptides. Advanced stage disease patients were more likely to be seropositive (P = 0.00024). Biopsy specimens showed CK8 expression in all 24 cases by immunochemistry and CK18 in 22 cases. This is the first study to demonstrate that a subgroup of NSCLC patients have intact CK8 and CK18 peptides in their serum, and their detection may correlate with advanced disease. {\textcopyright} 1994 Elsevier Science Ltd.",

author = "N. Pendleton and Occleston, {N. L.} and Walshaw, {M. J.} and Myskow, {M. W.} and Littler, {J. A H} and Green, {J. A.}",

year = "1994",

language = "English",

volume = "30",

pages = "93--96",

journal = "European Journal of Cancer",

issn = "0959-8049",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - Simple cytokeratins in the serum of patients with lung cancer: Relationship to cell death

AU - Pendleton, N.

AU - Occleston, N. L.

AU - Walshaw, M. J.

AU - Myskow, M. W.

AU - Littler, J. A H

AU - Green, J. A.

PY - 1994

Y1 - 1994

N2 - An important role in differentiation and proliferation has been demonstrated for the 20 cytokeratin (CK) polypeptides. The serum of 24 patients with biopsy-proven non-small cell lung cancer (NSCLC) and a similar number of controls was examined for evidence of CK8 and CK18. Using enzyme-linked immunosorbent assay (ELISA), all the control sera were negative, but 9 of the 24 patients were positive (mean 2.62 ng/ml; range 1.4-5.8; P = 0.0036). Western blotting confirmed the results of the ELISA in all cases, and indicated full size CK polypeptides. Advanced stage disease patients were more likely to be seropositive (P = 0.00024). Biopsy specimens showed CK8 expression in all 24 cases by immunochemistry and CK18 in 22 cases. This is the first study to demonstrate that a subgroup of NSCLC patients have intact CK8 and CK18 peptides in their serum, and their detection may correlate with advanced disease. © 1994 Elsevier Science Ltd.

AB - An important role in differentiation and proliferation has been demonstrated for the 20 cytokeratin (CK) polypeptides. The serum of 24 patients with biopsy-proven non-small cell lung cancer (NSCLC) and a similar number of controls was examined for evidence of CK8 and CK18. Using enzyme-linked immunosorbent assay (ELISA), all the control sera were negative, but 9 of the 24 patients were positive (mean 2.62 ng/ml; range 1.4-5.8; P = 0.0036). Western blotting confirmed the results of the ELISA in all cases, and indicated full size CK polypeptides. Advanced stage disease patients were more likely to be seropositive (P = 0.00024). Biopsy specimens showed CK8 expression in all 24 cases by immunochemistry and CK18 in 22 cases. This is the first study to demonstrate that a subgroup of NSCLC patients have intact CK8 and CK18 peptides in their serum, and their detection may correlate with advanced disease. © 1994 Elsevier Science Ltd.

M3 - Article

SN - 0959-8049

VL - 30

SP - 93

EP - 96

JO - European Journal of Cancer

JF - European Journal of Cancer

IS - 1

ER -

Simple cytokeratins in the serum of patients with lung cancer: Relationship to cell death

Abstract

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