Simple leaky-waveguide devices for the detection of bacterial spores

John P. Hulme; Nicholas J. Goddard; Chen Lu

doi:10.1016/j.snb.2011.09.076

Simple leaky-waveguide devices for the detection of bacterial spores

John P. Hulme, Nicholas J. Goddard, Chen Lu

Research output: Contribution to journal › Article › peer-review

Abstract

A variety of biocompatible optical leaky waveguide sensors were developed for bacterial detection. Divalent sensitive polymer waveguides incorporating the chelating agent nitrilotriacetic acid (NTA) were used to monitor the release of Ca2+ ions from Bacillus subtilis spores during the first stage of germination. Co-polymer waveguides incorporating hydrolyzed starch were used to detect the presence of exo-enzymes released during the later stages of vegetative growth. The limit of detection for calcium, based on changes in the intensity of the TE0 mode was 60 μM. Changes in refractive index were demonstrated by measuring the change in the angular position of the TE 1 mode resulting from the degradation of the waveguide by α-amylase. © 2011 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	1508-1513
Number of pages	5
Journal	Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2011.09.076
Publication status	Published - 15 Dec 2011

Keywords

HEMA
Label free
Leaky
Low index
Waveguide

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10.1016/j.snb.2011.09.076

http://www.sciencedirect.com/science/article/pii/S0925400511008781

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abstract = "A variety of biocompatible optical leaky waveguide sensors were developed for bacterial detection. Divalent sensitive polymer waveguides incorporating the chelating agent nitrilotriacetic acid (NTA) were used to monitor the release of Ca2+ ions from Bacillus subtilis spores during the first stage of germination. Co-polymer waveguides incorporating hydrolyzed starch were used to detect the presence of exo-enzymes released during the later stages of vegetative growth. The limit of detection for calcium, based on changes in the intensity of the TE0 mode was 60 μM. Changes in refractive index were demonstrated by measuring the change in the angular position of the TE 1 mode resulting from the degradation of the waveguide by α-amylase. {\textcopyright} 2011 Elsevier B.V. All rights reserved.",

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AU - Goddard, Nicholas J.

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KW - Waveguide

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M3 - Article

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JO - Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers

JF - Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers

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ER -

Simple leaky-waveguide devices for the detection of bacterial spores

Abstract

Keywords

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