Simple staining detects ultrastructural and biochemical differentiation of vegetative hyphae and fruit body initials in colonies of Pleurotus pulmonarius

C. Sánchez, M. Téllez-Téllez, G. Díaz-Godínez, D. Moore

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Aims: To know the ultrastructural and biochemical differences of vegetative hyphae and fruit body initials in colonies of Pleurotus pulmonarius. Methods and Results: Feulgen reagent was used to detects differentiation of hyphae. The intracellular laccases, proteases and β-1,3-glucanases activity, content of cytoplasmic protein, glycogen and glucans in the cell wall were evaluated in hyphae of fruit body initials and in vegetative hyphae. The thickness of hyphal walls of the vegetative hyphae was also evaluated. Substantial biochemical changes were observed in hyphae of different zones of the fruiting colony. Hyphae at the periphery had thinner walls than in the centre of the colony. Conclusion, Significance and Impact of the Study: Staining correlated with the enzymatic activity, protein, glycogen and glucans, in mycelium and in fruit body initials. The implications are that hyphal maturity in P. pulmonarius involves storage of glucans, in part at least, in the form of a thickened hyphal wall.
    Original languageEnglish
    Pages (from-to)483-487
    Number of pages4
    JournalLetters in Applied Microbiology
    Volume38
    Issue number6
    DOIs
    Publication statusPublished - 2004

    Keywords

    • Basidiomycetes
    • Enzymatic activity
    • Fruit body initials
    • Hyphal differentiation
    • Vegetative mycelium

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