Single nisoldipine-sensitive calcium channels in smooth muscle cells isolated from rabbit mesenteric artery

J. F. Worley, J. W. Deitmer, M. T. Nelson

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Single smooth muscle cells were enzymatically isolated from the rabbit mesenteric artery. At physiological levels of external Ca, these cells were relaxed and contracted on exposure to norepinephrine, caffeine, or high levels of potassium. The patch-clamp technique was used to measure unitary currents through single channels in the isolated cells. Single channels were selective for divalent cations and exhibited two conductance levels, 8 pS and 15 pS. Both types of channels were voltage-dependent, and channel activity occurred at potentials positive to -40 mV. The activity of both channel types was almost completely inhibited by 50 nM nisoldipine. These channels appear to be the pathways for voltage-dependent Ca influx in vascular smooth muscle and may be the targets of the clinically used dihydropyridines.
    Original languageEnglish
    Pages (from-to)5746-5750
    Number of pages4
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume83
    Issue number15
    Publication statusPublished - 1986

    Keywords

    • Animals
    • physiology: Calcium
    • Cell Separation
    • Electric Conductivity
    • drug effects: Ion Channels
    • Membrane Potentials
    • Mesenteric Arteries
    • drug effects: Muscle, Smooth, Vascular
    • analogs & derivatives: Nifedipine
    • Nisoldipine
    • Rabbits
    • physiology: Sarcolemma

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