siRNA for gene silencing: A route to drug target discovery

Simon W. Jones; Patricia M. de Souza; Mark A. Lindsay

doi:10.1016/j.coph.2004.06.003

siRNA for gene silencing: A route to drug target discovery

Simon W. Jones, Patricia M. de Souza, Mark A. Lindsay

Research output: Contribution to journal › Article › peer-review

Abstract

The identification of RNA interference in mammalian cells, mediated via both virally-derived short interference RNA (siRNA) and endogenously produced microRNA, has revolutionised our understanding of the translational control of gene expression. Indeed, since its initial discovery, siRNA has been rapidly deployed for the elucidation of gene function and the identification of potential drug targets, a process often known as target discovery. In this review, we briefly discuss the mechanism of RNA interference and then critically examine the use of siRNA in target discovery, with a particular emphasis upon issues such as efficacy, selectivity, delivery and application in high-throughput studies. © 2004 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	522-527
Number of pages	5
Journal	Current opinion in pharmacology
Volume	4
Issue number	5
DOIs	https://doi.org/10.1016/j.coph.2004.06.003
Publication status	Published - Oct 2004

Keywords

Animals
Gene Silencing
Gene Transfer Techniques
Humans
physiology: RNA Interference
genetics: RNA, Small Interfering

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10.1016/j.coph.2004.06.003

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title = "siRNA for gene silencing: A route to drug target discovery",

abstract = "The identification of RNA interference in mammalian cells, mediated via both virally-derived short interference RNA (siRNA) and endogenously produced microRNA, has revolutionised our understanding of the translational control of gene expression. Indeed, since its initial discovery, siRNA has been rapidly deployed for the elucidation of gene function and the identification of potential drug targets, a process often known as target discovery. In this review, we briefly discuss the mechanism of RNA interference and then critically examine the use of siRNA in target discovery, with a particular emphasis upon issues such as efficacy, selectivity, delivery and application in high-throughput studies. {\textcopyright} 2004 Elsevier Ltd. All rights reserved.",

keywords = "Animals, Gene Silencing, Gene Transfer Techniques, Humans, physiology: RNA Interference, genetics: RNA, Small Interfering",

author = "Jones, {Simon W.} and {de Souza}, {Patricia M.} and Lindsay, {Mark A.}",

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T1 - siRNA for gene silencing: A route to drug target discovery

AU - Jones, Simon W.

AU - de Souza, Patricia M.

AU - Lindsay, Mark A.

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N2 - The identification of RNA interference in mammalian cells, mediated via both virally-derived short interference RNA (siRNA) and endogenously produced microRNA, has revolutionised our understanding of the translational control of gene expression. Indeed, since its initial discovery, siRNA has been rapidly deployed for the elucidation of gene function and the identification of potential drug targets, a process often known as target discovery. In this review, we briefly discuss the mechanism of RNA interference and then critically examine the use of siRNA in target discovery, with a particular emphasis upon issues such as efficacy, selectivity, delivery and application in high-throughput studies. © 2004 Elsevier Ltd. All rights reserved.

AB - The identification of RNA interference in mammalian cells, mediated via both virally-derived short interference RNA (siRNA) and endogenously produced microRNA, has revolutionised our understanding of the translational control of gene expression. Indeed, since its initial discovery, siRNA has been rapidly deployed for the elucidation of gene function and the identification of potential drug targets, a process often known as target discovery. In this review, we briefly discuss the mechanism of RNA interference and then critically examine the use of siRNA in target discovery, with a particular emphasis upon issues such as efficacy, selectivity, delivery and application in high-throughput studies. © 2004 Elsevier Ltd. All rights reserved.

KW - Animals

KW - Gene Silencing

KW - Gene Transfer Techniques

KW - Humans

KW - physiology: RNA Interference

KW - genetics: RNA, Small Interfering

U2 - 10.1016/j.coph.2004.06.003

DO - 10.1016/j.coph.2004.06.003

M3 - Article

SN - 1471-4892

VL - 4

SP - 522

EP - 527

JO - Current opinion in pharmacology

JF - Current opinion in pharmacology

IS - 5

ER -

siRNA for gene silencing: A route to drug target discovery

Abstract

Keywords

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