Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

S. Piluso; H.C. Cassell; J.L. Gibbons; T.E. Waller; N.J. Plant; A.F. Miller; G. Cavalli

doi:10.1039/c3sm00131h

Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

S. Piluso, H.C. Cassell, J.L. Gibbons, T.E. Waller, N.J. Plant, A.F. Miller, G. Cavalli

CE - Academic & Research

Research output: Contribution to journal › Article › peer-review

Abstract

The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.

Original language	English
Pages (from-to)	6752-6756
Number of pages	5
Journal	Soft Matter
Volume	9
Issue number	29
Early online date	7 Mar 2013
DOIs	https://doi.org/10.1039/c3sm00131h
Publication status	Published - 7 Aug 2013

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AU - Piluso, S.

AU - Cassell, H.C.

AU - Gibbons, J.L.

AU - Waller, T.E.

AU - Plant, N.J.

AU - Miller, A.F.

AU - Cavalli, G.

PY - 2013/8/7

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AB - The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.

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U2 - 10.1039/c3sm00131h

DO - 10.1039/c3sm00131h

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Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

Abstract

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