Sol-gel synthesis at neutral pH in W/O microemulsion: A method for enzyme nanoencapsulation in silica gel nanoparticles

F. Cellesi; N. Tirelli

doi:10.1016/j.colsurfa.2006.05.008

Sol-gel synthesis at neutral pH in W/O microemulsion: A method for enzyme nanoencapsulation in silica gel nanoparticles

F. Cellesi, N. Tirelli

Research output: Contribution to journal › Article › peer-review

Abstract

The classical sol-gel synthesis of silica gels has been adapted to a W/O (micro)emulsion process under conditions that minimize denaturing effects on encapsulated enzymes. We have in particular focused on optimizing the purification procedures with the aim to produce water nanoparticles dispersions from W/O emulsions without the use of precipitation/sedimentation steps. A proof of principle of encapsulation has been conducted using horseradish peroxidase (HRP). Crown Copyright © 2006.

Original language	English
Pages (from-to)	52-61
Number of pages	9
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	288
Issue number	1-3
DOIs	https://doi.org/10.1016/j.colsurfa.2006.05.008
Publication status	Published - 5 Oct 2006

Keywords

Enzymes
Nanoparticles
Silica
Sol-gel

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10.1016/j.colsurfa.2006.05.008

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abstract = "The classical sol-gel synthesis of silica gels has been adapted to a W/O (micro)emulsion process under conditions that minimize denaturing effects on encapsulated enzymes. We have in particular focused on optimizing the purification procedures with the aim to produce water nanoparticles dispersions from W/O emulsions without the use of precipitation/sedimentation steps. A proof of principle of encapsulation has been conducted using horseradish peroxidase (HRP). Crown Copyright {\textcopyright} 2006.",

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AU - Tirelli, N.

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N2 - The classical sol-gel synthesis of silica gels has been adapted to a W/O (micro)emulsion process under conditions that minimize denaturing effects on encapsulated enzymes. We have in particular focused on optimizing the purification procedures with the aim to produce water nanoparticles dispersions from W/O emulsions without the use of precipitation/sedimentation steps. A proof of principle of encapsulation has been conducted using horseradish peroxidase (HRP). Crown Copyright © 2006.

AB - The classical sol-gel synthesis of silica gels has been adapted to a W/O (micro)emulsion process under conditions that minimize denaturing effects on encapsulated enzymes. We have in particular focused on optimizing the purification procedures with the aim to produce water nanoparticles dispersions from W/O emulsions without the use of precipitation/sedimentation steps. A proof of principle of encapsulation has been conducted using horseradish peroxidase (HRP). Crown Copyright © 2006.

KW - Enzymes

KW - Nanoparticles

KW - Silica

KW - Sol-gel

U2 - 10.1016/j.colsurfa.2006.05.008

DO - 10.1016/j.colsurfa.2006.05.008

M3 - Article

SN - 0927-7757

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JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

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ER -

Sol-gel synthesis at neutral pH in W/O microemulsion: A method for enzyme nanoencapsulation in silica gel nanoparticles

Abstract

Keywords

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