We report the solution structure of the carbohydrate-binding B subunit of verotoxin VT-1 (VTB) from enterohemorrhagic Escherichia coli in association with the trisaccharide Galα1−4Galβ1−4Glcβ1-O-trimethylsilylethyl, determined by use of stable isotope-assisted NMR techniques. In contrast to the crystal structure of the complex which predicts three binding sites per monomer, only one of these sites is observed with substantial occupancy by the trisaccharide in solution.
- Crystal structure
- Screening assays
- Resonance structures
Research Beacons, Institutes and Platforms
- Manchester Institute of Biotechnology