Solution structure of the complex between the B-subunit homopentamer of verotoxin VT-1 from Escherichia coli and the trisaccharide moiety of globotriaosylceramide

H. Shimizu, R.A. Field, S.W. Homans, A. Donohue-Rolfe

Research output: Contribution to journalArticlepeer-review


We report the solution structure of the carbohydrate-binding B subunit of verotoxin VT-1 (VTB) from enterohemorrhagic Escherichia coli in association with the trisaccharide Galα1−4Galβ1−4Glcβ1-O-trimethylsilylethyl, determined by use of stable isotope-assisted NMR techniques. In contrast to the crystal structure of the complex which predicts three binding sites per monomer, only one of these sites is observed with substantial occupancy by the trisaccharide in solution.
Original languageUndefined
Pages (from-to)11078-11082
Number of pages5
Issue number31
Publication statusPublished - 16 Jul 1998


  • Hydroxyls
  • Ligands
  • Crystal structure
  • Screening assays
  • Resonance structures

Research Beacons, Institutes and Platforms

  • Manchester Institute of Biotechnology

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