Solvent-slaved protein motions accompany proton coupled electron transfer reactions catalysed by copper nitrite reductase

Tobias Hedison; Derren Heyes; Muralidharan Shanmugam; Andreea Iulia Iorgu; Nigel Scrutton

doi:10.1039/C9CC01026B

Solvent-slaved protein motions accompany proton coupled electron transfer reactions catalysed by copper nitrite reductase

Tobias Hedison, Derren Heyes, Muralidharan Shanmugam, Andreea Iulia Iorgu, Nigel Scrutton

Division of Molecular & Cellular Function (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

Through the use of time-resolved pH-jump spectroscopy, we demonstrate how proton transfer is coupled to inter-copper electron transfer in a copper nitrite reductase (CuNiR). Combined use of electron paramagnetic resonance spectroscopy with solvent viscosity- and pressure-dependence pH-jump stopped-flow spectroscopy is used to show that solvent-slaved protein motions are linked to this proton coupled electron transfer step in CuNiR.

Original language	English
Journal	Chemical Communications
DOIs	https://doi.org/10.1039/C9CC01026B
Publication status	Published - 3 May 2019

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Manchester Institute of Biotechnology

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@article{ffb72c6691864ca69ab71cb2b90eab7b,

title = "Solvent-slaved protein motions accompany proton coupled electron transfer reactions catalysed by copper nitrite reductase",

abstract = "Through the use of time-resolved pH-jump spectroscopy, we demonstrate how proton transfer is coupled to inter-copper electron transfer in a copper nitrite reductase (CuNiR). Combined use of electron paramagnetic resonance spectroscopy with solvent viscosity- and pressure-dependence pH-jump stopped-flow spectroscopy is used to show that solvent-slaved protein motions are linked to this proton coupled electron transfer step in CuNiR.",

author = "Tobias Hedison and Derren Heyes and Muralidharan Shanmugam and Iorgu, {Andreea Iulia} and Nigel Scrutton",

year = "2019",

month = may,

day = "3",

doi = "10.1039/C9CC01026B",

language = "English",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Solvent-slaved protein motions accompany proton coupled electron transfer reactions catalysed by copper nitrite reductase

AU - Hedison, Tobias

AU - Heyes, Derren

AU - Shanmugam, Muralidharan

AU - Iorgu, Andreea Iulia

AU - Scrutton, Nigel

PY - 2019/5/3

Y1 - 2019/5/3

N2 - Through the use of time-resolved pH-jump spectroscopy, we demonstrate how proton transfer is coupled to inter-copper electron transfer in a copper nitrite reductase (CuNiR). Combined use of electron paramagnetic resonance spectroscopy with solvent viscosity- and pressure-dependence pH-jump stopped-flow spectroscopy is used to show that solvent-slaved protein motions are linked to this proton coupled electron transfer step in CuNiR.

AB - Through the use of time-resolved pH-jump spectroscopy, we demonstrate how proton transfer is coupled to inter-copper electron transfer in a copper nitrite reductase (CuNiR). Combined use of electron paramagnetic resonance spectroscopy with solvent viscosity- and pressure-dependence pH-jump stopped-flow spectroscopy is used to show that solvent-slaved protein motions are linked to this proton coupled electron transfer step in CuNiR.

U2 - 10.1039/C9CC01026B

DO - 10.1039/C9CC01026B

M3 - Article

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

ER -

Solvent-slaved protein motions accompany proton coupled electron transfer reactions catalysed by copper nitrite reductase

Abstract

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