Species differentiation by internally transcribed spacer PCR and HhaI digestion of fluconazole-resistant Candida krusei, Candida inconspicua, and Candida norvegensis strains

Seonghun Nho, Michael J. Anderson, Caroline B. Moore, David W. Denning

Research output: Contribution to journalArticlepeer-review

Abstract

PCR amplification of the regions containing the internally transcribed spacers and 5.8S rRNA gene of Candida krusei, C. inconspicua, and C. norvegensis yielded fragments of 510, 460, and 500 bp, respectively. HhaI digestion of these fragments yielded species-specific bands. Random amplification of polymorphic DNA with primer R108 showed interspecific discriminatory band patterns. Susceptibilities to fluconazole and amphotericin B were determined.

Original languageEnglish
Pages (from-to)1036-1039
Number of pages4
JournalJournal of clinical microbiology
Volume35
Issue number4
Publication statusPublished - 1 Apr 1997

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