Specific neuro-radiological diagnosis of Herpes encephalitis in an animal model

G. M. Cleator; P. E. Klapper; A. G. Lewis; H. L. Sharma; M. Longson

doi:10.1007/BF01314647

Specific neuro-radiological diagnosis of Herpes encephalitis in an animal model

G. M. Cleator, P. E. Klapper, A. G. Lewis, H. L. Sharma, M. Longson

Research output: Contribution to journal › Article › peer-review

Abstract

The potential of utilizing a radio-labelled derivative of the antiviral drug (E)-5-(2-iodovinyl)-2′-deoxyuridine (IVDU) for the specific, non-invasive, in vivo diagnosis of Herpes simplex virus encephalitis (HSVE) was investigated in a rat model of the disease. Following pharmacological disruption of the blood brain barrier radiolabelled IVDU was administered by intra-carotid injection. Brain radioactivity was compared between control and infected animals via gamma camera scintigraphy. After clearance of non-metabolized drug, markedly higher levels of activity were found in infected brain. Post-mortem studies of cryostat sections of brain examined by autoradiography and immunochemical staining showed the radioactivity selectively accumulated in areas of virus infection. These results indicate that radio-labelled derivatives of antiviral drugs may allow the specific neuro-radiological diagnosis of HSVE. © 1988 Springer-Verlag.

Original language	English
Pages (from-to)	1-12
Number of pages	11
Journal	Archives of Virology
Volume	101
Issue number	1-2
DOIs	https://doi.org/10.1007/BF01314647
Publication status	Published - Mar 1988

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title = "Specific neuro-radiological diagnosis of Herpes encephalitis in an animal model",

abstract = "The potential of utilizing a radio-labelled derivative of the antiviral drug (E)-5-(2-iodovinyl)-2′-deoxyuridine (IVDU) for the specific, non-invasive, in vivo diagnosis of Herpes simplex virus encephalitis (HSVE) was investigated in a rat model of the disease. Following pharmacological disruption of the blood brain barrier radiolabelled IVDU was administered by intra-carotid injection. Brain radioactivity was compared between control and infected animals via gamma camera scintigraphy. After clearance of non-metabolized drug, markedly higher levels of activity were found in infected brain. Post-mortem studies of cryostat sections of brain examined by autoradiography and immunochemical staining showed the radioactivity selectively accumulated in areas of virus infection. These results indicate that radio-labelled derivatives of antiviral drugs may allow the specific neuro-radiological diagnosis of HSVE. {\textcopyright} 1988 Springer-Verlag.",

author = "Cleator, {G. M.} and Klapper, {P. E.} and Lewis, {A. G.} and Sharma, {H. L.} and M. Longson",

year = "1988",

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T1 - Specific neuro-radiological diagnosis of Herpes encephalitis in an animal model

AU - Cleator, G. M.

AU - Klapper, P. E.

AU - Lewis, A. G.

AU - Sharma, H. L.

AU - Longson, M.

PY - 1988/3

Y1 - 1988/3

N2 - The potential of utilizing a radio-labelled derivative of the antiviral drug (E)-5-(2-iodovinyl)-2′-deoxyuridine (IVDU) for the specific, non-invasive, in vivo diagnosis of Herpes simplex virus encephalitis (HSVE) was investigated in a rat model of the disease. Following pharmacological disruption of the blood brain barrier radiolabelled IVDU was administered by intra-carotid injection. Brain radioactivity was compared between control and infected animals via gamma camera scintigraphy. After clearance of non-metabolized drug, markedly higher levels of activity were found in infected brain. Post-mortem studies of cryostat sections of brain examined by autoradiography and immunochemical staining showed the radioactivity selectively accumulated in areas of virus infection. These results indicate that radio-labelled derivatives of antiviral drugs may allow the specific neuro-radiological diagnosis of HSVE. © 1988 Springer-Verlag.

AB - The potential of utilizing a radio-labelled derivative of the antiviral drug (E)-5-(2-iodovinyl)-2′-deoxyuridine (IVDU) for the specific, non-invasive, in vivo diagnosis of Herpes simplex virus encephalitis (HSVE) was investigated in a rat model of the disease. Following pharmacological disruption of the blood brain barrier radiolabelled IVDU was administered by intra-carotid injection. Brain radioactivity was compared between control and infected animals via gamma camera scintigraphy. After clearance of non-metabolized drug, markedly higher levels of activity were found in infected brain. Post-mortem studies of cryostat sections of brain examined by autoradiography and immunochemical staining showed the radioactivity selectively accumulated in areas of virus infection. These results indicate that radio-labelled derivatives of antiviral drugs may allow the specific neuro-radiological diagnosis of HSVE. © 1988 Springer-Verlag.

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DO - 10.1007/BF01314647

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VL - 101

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JO - Archives of Virology

JF - Archives of Virology

IS - 1-2

ER -

Specific neuro-radiological diagnosis of Herpes encephalitis in an animal model

Abstract

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