Spectrometer for laser-light scattering measurements of macromolecular and subcellular particle motions

D. B. Sattelle, G. R. Palmer, M. C A Griffin, R. E D Holder

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The design and construction of a laser light scattering spectrometer suitable for diffusion and flow measurements on biological materials is described. The type of quantitative information that can be obtained using this instrument in conjunction with photon correlation signal processing includes: the diffusion coefficient of a macromolecule; the average size and dispersity of a sample of subcellular particles in vitro; the average velocity profile of streaming cytoplasm in vivo. The turntable unit and readily adjustable collection optics enable a variety of samples to be investigated at a range of scattering angles.
    Original languageEnglish
    Pages (from-to)37-43
    Number of pages6
    JournalMedical and Biological Engineering and Computing
    Volume20
    Issue number1
    Publication statusPublished - Jan 1982

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