@inbook{7e9e492804284ee39a6ec9974bf734ef,
title = "SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution",
abstract = "Directed evolution of proteins is a highly effective strategy for tailoring biocatalysts to a particular application, and is capable of engineering improvements such as kcat, thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein{\textquoteright}s vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address this, we have developed SpeedyGenesXL, an automated, high-throughput platform for the production of wild-type genes, Boolean OR, combinatorial, or combinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.",
keywords = "Automation, Bespoke gene variant library synthesis, Directed evolution, Synthetic biology",
author = "Sadler, {Joanna C.} and Neil Swainston and Dunstan, {Mark S.} and Andrew Currin and Kell, {Douglas B.}",
note = "Funding Information: JCS thanks the SYNBIOCHEM center for use of equipment and the BBSRC (grants BBSRC BB/M017702/1). DBK thanks the BBSRC (grants BB/P009042/1 and BB/R000093/1), the Novo Nordisk Fonden via the Centre for Biosustainability (grant NNF20CC0035580), and the University of Liverpool for financial support. Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2152-3_5",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press, Inc",
pages = "67--83",
booktitle = "Methods in Molecular Biology",
address = "United States",
}