SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution

Joanna C. Sadler, Neil Swainston, Mark S. Dunstan, Andrew Currin, Douglas B. Kell

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Directed evolution of proteins is a highly effective strategy for tailoring biocatalysts to a particular application, and is capable of engineering improvements such as kcat, thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein’s vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address this, we have developed SpeedyGenesXL, an automated, high-throughput platform for the production of wild-type genes, Boolean OR, combinatorial, or combinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press, Inc
Pages67-83
Number of pages17
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2461
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Automation
  • Bespoke gene variant library synthesis
  • Directed evolution
  • Synthetic biology

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