Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions

Zhenqiang Zhao, Zhibin Chen, Xiubo Zhao, Fang Pan, Meihua Cai, Tan Wang, Henggui Zhang, Jian R. Lu, Ming Lei

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Background: It is of growing interest to develop novel approaches to initiate differentiation of mesenchymal stem cells (MSCs) into cardiomyocytes. The purpose of this investigation was to determine if Sphingosine-1-phosphate (S1P), a native circulating bioactive lipid metabolite, plays a role in differentiation of human umbilical cord mesenchymal stem cells (HUMSCs) into cardiomyocytes. We also developed an engineered cell sheet from these HUMSCs derived cardiomyocytes by using a temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm) cell sheet technology. Methods. Cardiomyogenic differentiation of HUMSCs was performed by culturing these cells with either designated cardiomyocytes conditioned medium (CMCM) alone, or with 1 M S1P; or DMEM with 10% FBS + 1 M S1P. Cardiomyogenic differentiation was determined by immunocytochemical analysis of expression of cardiomyocyte markers and patch clamping recording of the action potential. Results: A cardiomyocyte-like morphology and the expression of -actinin and myosin heavy chain (MHC) proteins can be observed in both CMCM culturing or CMCM+S1P culturing groups after 5 days' culturing, however, only the cells in CMCM+S1P culture condition present cardiomyocyte-like action potential and voltage gated currents. A new approach was used to form PIPAAm based temperature-responsive culture surfaces and this successfully produced cell sheets from HUMSCs derived cardiomyocytes. Conclusions: This study for the first time demonstrates that S1P potentiates differentiation of HUMSCs towards functional cardiomyocytes under the designated culture conditions. Our engineered cell sheets may provide a potential for clinically applicable myocardial tissues should promote cardiac tissue engineering research. © 2011 Zhao et al; licensee BioMed Central Ltd.
    Original languageEnglish
    Article number37
    JournalJournal of Biomedical Science
    Volume18
    Issue number1
    DOIs
    Publication statusPublished - 2011

    Keywords

    • engineered cell sheets
    • sphingosine-1-phosphate
    • umbilical cord mesenchymal stem cells

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