Squeezing, Hearing and Illuminating Prostate Cancer: Searching for Diagnostic Signatures

Elsa Correia Faria - bill, Faria E. Correia, T. J. Harvey, C. Hughes, A. D. Ward, A. Henderson, N. W. Clarke, M. D. Brown, R. D. Snook, P. Gardner

    Research output: Contribution to conferencePoster

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    Abstract

    Squeezing, Hearing and Illuminating Prostate Cancer Cells - Searching for Diagnostic SignaturesCurrently, the diagnosis of prostate cancer is achieved by annual PSA screening and digital rectal examination (DRE). However, the lack of sensitivity and specificity of PSA as a tool to diagnose prostate cancer, as well as its inability to predict the clinical aggressiveness of a tumour have limited its utility. In our work we search for diagnostic signatures for prostate cancer which might also provide information on the aggressiveness of the cancer. To this end we have used reflection mode Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS), Raman Laser Tweezers1 and atomic force microscopy (AFM) to study the applicability of these techniques to discriminate between prostate cancer cells of differing invasiveness and non-cancer prostate cells. Results of our work have shown that the cells can be discriminated spectroscopically using FTIR-PAS2 and Raman Laser Tweezers.3, 4 Sensitivities and specificities of >93% and 98% were achieved using Raman Laser Tweezers and Principal component-linear discriminate analysis (PCLDA). In addition, we have investigated the hypothesis that the mechanical properties of cells might be a useful marker for cancer progression. It has been suggested that cancer cells are less stiff than non-cancer cells and that invasive cancer cells are less stiff than non-invasive cancer cells. We have determined the stiffness of non-cancerous prostate cells (BPH), non-invasive prostate cancer cells (LNCaP) and highly invasive prostate cancer cells (PC-3) using an atomic force microscope (AFM).5 We have shown that prostate cancer cells were more easily deformed than non-cancer cells. However, the highly invasive PC-3 cells were stiffer than the non-invasive LNCaP.1. Snook R. D., Harvey T. J. , Correia Faria E. and Gardner P., Raman Tweezers and their Application to the Study of Singly Trapped Eukaryotic Cells, Integrative Biology, 2009, 1, 43-522. Harvey T. J., Henderson A., Gazi E., Clarke N. W., Brown M., Correia Faria E., Snook R. D. and Gardner P., Discrimination of Prostate Cancer Cells by Reflection Mode FTIR Photoacoustic Spectroscopy, The Analyst, 2007, 132, 292-2953. Harvey T. J., Hughes C., Ward A. D., Correia Faria E., Henderson A., Clarke N. W., Brown M. D., Snook R. D. and Gardner P., Classification of Fixed Urological Cells using Raman Tweezers, Journal of Biophotonics, 2009, 2, 47-694. Harvey T. J., Correia Faria E., Gazi E., Ward A. D., Clarke N. W., Brown M., Snook R. D. and Gardner P., A Preliminary Investigation into the Spectral Discrimination of Live Prostate and Bladder Cancer Cell Lines Using Raman Optical Tweezers, Journal of Biomedical Optics., 2008, 13, 064004–0640115. Correia Faria E., Ma N., Gazi E., Gardner P., Brown M., Clarke N. W. and Snook R. D., Measurement of Elastic Properties of Prostate Cancer Cells using AFM, The Analyst, 2008, 133, 1498-500.
    Original languageEnglish
    Publication statusPublished - Jun 2010
    EventSPEC2010 "Shedding Light on Disease: Optical Diagnosis for the new Millenium" - University of Manchester
    Duration: 26 Jun 20101 Jul 2010

    Conference

    ConferenceSPEC2010 "Shedding Light on Disease: Optical Diagnosis for the new Millenium"
    CityUniversity of Manchester
    Period26/06/101/07/10

    Keywords

    • Prostate Cancer
    • diagnostic
    • Raman
    • Laser Tweezers
    • Optical Tweezers
    • Atomic Force Microscopy
    • AFM
    • Cell elasticity
    • Young's Modulus
    • Photoacoustic spectroscopy
    • PC-LDA

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