Stable Plastid Transformation of Petunia for Studies in Basic Research

Elena Martin Avila, Anil Day

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Petunia hybrida is a commercial ornamental plant and is also an important model species for genetic analysis 6 and transgenic research. Here we describe the steps required to isolate stable plastid transformants in P. hybrida using the commercial Pink Wave cultivar. Wave cultivars are popular spreading Petunias sold as 8 ground cover and potted plants. Transgenes introduced into P. hybrida plastids exhibit stable expression over many generations. The development of plastid transformation in P. hybrida provides an enabling technology to bring the benefits of plastid engineering, including maternal inheritance and stable expression of performance-enhancing trait genes, to the important floriculture and horticulture industries.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationChloroplast Biotechnology
EditorsPal Maliga
Number of pages17
ISBN (Electronic)978-1-0716-1472-3
Publication statusPublished - 25 May 2021

Publication series

NameMethods in Molecular Biology
PublisherHumana, New York


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