Staining Fission Yeast Filamentous Actin with Fluorescent Phalloidin Conjugates

Iain Hagan

doi:10.1101/pdb.prot091033

Staining Fission Yeast Filamentous Actin with Fluorescent Phalloidin Conjugates

Iain Hagan

Cancer Research UK Manchester Institute

Research output: Contribution to journal › Article › peer-review

Abstract

The Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently conjugated phalloidin. Simultaneous staining of cell wall landmarks (with calcofluor) and chromatin (with 4',6-diamidino-2-phenylindole, or DAPI) makes this rapid staining procedure highly effective for staging cell cycle progression, monitoring morphogenetic abnormalities, and assessing the impact of environmental and genetic changes on the integrity of the F-actin cytoskeleton.

Original language	English
Article number	pdb.prot091033
Journal	Cold Spring Harbor Protocols
Volume	2016
Issue number	6
DOIs	https://doi.org/10.1101/pdb.prot091033
Publication status	Published - Jun 2016

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Manchester Cancer Research Centre

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abstract = "The Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently conjugated phalloidin. Simultaneous staining of cell wall landmarks (with calcofluor) and chromatin (with 4',6-diamidino-2-phenylindole, or DAPI) makes this rapid staining procedure highly effective for staging cell cycle progression, monitoring morphogenetic abnormalities, and assessing the impact of environmental and genetic changes on the integrity of the F-actin cytoskeleton.",

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AB - The Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently conjugated phalloidin. Simultaneous staining of cell wall landmarks (with calcofluor) and chromatin (with 4',6-diamidino-2-phenylindole, or DAPI) makes this rapid staining procedure highly effective for staging cell cycle progression, monitoring morphogenetic abnormalities, and assessing the impact of environmental and genetic changes on the integrity of the F-actin cytoskeleton.

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Staining Fission Yeast Filamentous Actin with Fluorescent Phalloidin Conjugates

Abstract

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