Stimulus-specific hierarchy of enhancer elements within the rat prodynorphin promoter

Angel M. Carrión, Britt Mellström, Simon M. Luckman, Jose R. Naranjo

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Induction of the prodynorphin gene occurs in a tissue-specific manner following different physiological stimuli. Using electrophoretic mobility shift assays, we studied the relative activity of the five major regulatory sites in the rat prodynorphin promoter. Prodynorphin cyclic AMP-responsive element 2 (DynCRE2), DynCRE3, and the noncanonical prodynorphin AP-1 (ncDynAP-1) regulatory sites control, in a coordinated manner, prodynorphin induction in the spinal cord after noxious stimulation, whereas prodynorphin up-regulation in supraoptic neurons is regulated predominantly by the ncDynAP-1. Conversely, prodynorphin transactivation in the ovaries upon gonadotropin stimulation is controlled by DynCRE1 and DynCRE3. Our results support the idea that stimulus-specific changes in nuclear proteins establish a functional hierarchy among regulatory sites in the prodynorphin promotor and provide further insight in the molecular mechanisms that govern prodynorphin gene regulation.
    Original languageEnglish
    Pages (from-to)914-921
    Number of pages7
    JournalJournal of neurochemistry
    Volume70
    Issue number3
    Publication statusPublished - Mar 1998

    Keywords

    • AP-1
    • Cyclic AMP
    • Cyclic AMP-responsive element binding protein
    • Transcription

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