Abstract
Induction of the prodynorphin gene occurs in a tissue-specific manner following different physiological stimuli. Using electrophoretic mobility shift assays, we studied the relative activity of the five major regulatory sites in the rat prodynorphin promoter. Prodynorphin cyclic AMP-responsive element 2 (DynCRE2), DynCRE3, and the noncanonical prodynorphin AP-1 (ncDynAP-1) regulatory sites control, in a coordinated manner, prodynorphin induction in the spinal cord after noxious stimulation, whereas prodynorphin up-regulation in supraoptic neurons is regulated predominantly by the ncDynAP-1. Conversely, prodynorphin transactivation in the ovaries upon gonadotropin stimulation is controlled by DynCRE1 and DynCRE3. Our results support the idea that stimulus-specific changes in nuclear proteins establish a functional hierarchy among regulatory sites in the prodynorphin promotor and provide further insight in the molecular mechanisms that govern prodynorphin gene regulation.
| Original language | English |
|---|---|
| Pages (from-to) | 914-921 |
| Number of pages | 7 |
| Journal | Journal of neurochemistry |
| Volume | 70 |
| Issue number | 3 |
| Publication status | Published - Mar 1998 |
Keywords
- AP-1
- Cyclic AMP
- Cyclic AMP-responsive element binding protein
- Transcription