Strong, specific, reversible binding ligands for transfer RNA: Comparison by fluorescence and NMR spectroscopies with distamycin binding for a new structural class of ligand

Elena V. Bichenkova, Seyed E. Sadat-Ebrahimi, Amanda N. Wilton, Niamh O'Toole, Debora S. Marks, Kenneth T. Douglas*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Binding data are presented for the interaction with brewer's yeast tRNA(Phe) of a new structural family of ligands, symmetrical bis- benzimidazoles. In addition specific perturbations in chemical shifts were detected by 1-dimensional NMR spectroscopy at 400 MHz for some imino and aromatic methyl protons of tRNA(Phe) when the tRNA was titrated with distamycin. Competitive displacement of the benzimidazole by added distamycin was followed fluorescence spectroscopy.

Original languageEnglish
Pages (from-to)1651-1665
Number of pages15
JournalNucleosides and Nucleotides
Volume17
Issue number9-11
DOIs
Publication statusPublished - 1998

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