Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods

Jerome H L Hui; Antonio Marco; Suzanne Hunt; Janet Melling; Sam Griffiths-Jones; Matthew Ronshaugen

doi:10.1093/nar/gks1445

Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods

Jerome H L Hui, Antonio Marco, Suzanne Hunt, Janet Melling, Sam Griffiths-Jones, Matthew Ronshaugen

Research output: Contribution to journal › Article › peer-review

Abstract

In Drosophila melanogaster, the iab-4/iab-8 locus encodes bi-directionally transcribed microRNAs that regulate the function of flanking Hox transcription factors. We show that bi-directional transcription, temporal and spatial expression patterns and Hox regulatory function of the iab-4/iab-8 locus are conserved between fly and the beetle Tribolium castaneum. Computational predictions suggest iab-4 and iab-8 microRNAs can target common sites, and cell-culture assays confirm that iab-4 and iab-8 function overlaps on Hox target sites in both fly and beetle. However, we observe key differences in the way Hox genes are targeted. For instance, abd-A transcripts are targeted only by iab-8 in Drosophila, whereas both iab-4 and iab-8 bind to Tribolium abd-A. Our evolutionary and functional characterization of a bi-directionally transcribed microRNA establishes the iab-4/iab-8 system as a model for understanding how multiple products from sense and antisense microRNAs target common sites. © The Author(s) 2013.

Original language	English
Pages (from-to)	3352-3361
Number of pages	9
Journal	Nucleic acids research.
Volume	41
Issue number	5
DOIs	https://doi.org/10.1093/nar/gks1445
Publication status	Published - Mar 2013

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10.1093/nar/gks1445Licence: CC BY

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title = "Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods",

abstract = "In Drosophila melanogaster, the iab-4/iab-8 locus encodes bi-directionally transcribed microRNAs that regulate the function of flanking Hox transcription factors. We show that bi-directional transcription, temporal and spatial expression patterns and Hox regulatory function of the iab-4/iab-8 locus are conserved between fly and the beetle Tribolium castaneum. Computational predictions suggest iab-4 and iab-8 microRNAs can target common sites, and cell-culture assays confirm that iab-4 and iab-8 function overlaps on Hox target sites in both fly and beetle. However, we observe key differences in the way Hox genes are targeted. For instance, abd-A transcripts are targeted only by iab-8 in Drosophila, whereas both iab-4 and iab-8 bind to Tribolium abd-A. Our evolutionary and functional characterization of a bi-directionally transcribed microRNA establishes the iab-4/iab-8 system as a model for understanding how multiple products from sense and antisense microRNAs target common sites. {\textcopyright} The Author(s) 2013.",

author = "Hui, {Jerome H L} and Antonio Marco and Suzanne Hunt and Janet Melling and Sam Griffiths-Jones and Matthew Ronshaugen",

note = "097820/Z/11/Z, Wellcome Trust, United KingdomBB/G011346/1, Biotechnology and Biological Sciences Research Council, United KingdomBB/H017801/1, Biotechnology and Biological Sciences Research Council, United Kingdom",

year = "2013",

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doi = "10.1093/nar/gks1445",

language = "English",

volume = "41",

pages = "3352--3361",

journal = "Nucleic acids research.",

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T1 - Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods

AU - Hui, Jerome H L

AU - Marco, Antonio

AU - Hunt, Suzanne

AU - Melling, Janet

AU - Griffiths-Jones, Sam

AU - Ronshaugen, Matthew

N1 - 097820/Z/11/Z, Wellcome Trust, United KingdomBB/G011346/1, Biotechnology and Biological Sciences Research Council, United KingdomBB/H017801/1, Biotechnology and Biological Sciences Research Council, United Kingdom

PY - 2013/3

Y1 - 2013/3

N2 - In Drosophila melanogaster, the iab-4/iab-8 locus encodes bi-directionally transcribed microRNAs that regulate the function of flanking Hox transcription factors. We show that bi-directional transcription, temporal and spatial expression patterns and Hox regulatory function of the iab-4/iab-8 locus are conserved between fly and the beetle Tribolium castaneum. Computational predictions suggest iab-4 and iab-8 microRNAs can target common sites, and cell-culture assays confirm that iab-4 and iab-8 function overlaps on Hox target sites in both fly and beetle. However, we observe key differences in the way Hox genes are targeted. For instance, abd-A transcripts are targeted only by iab-8 in Drosophila, whereas both iab-4 and iab-8 bind to Tribolium abd-A. Our evolutionary and functional characterization of a bi-directionally transcribed microRNA establishes the iab-4/iab-8 system as a model for understanding how multiple products from sense and antisense microRNAs target common sites. © The Author(s) 2013.

AB - In Drosophila melanogaster, the iab-4/iab-8 locus encodes bi-directionally transcribed microRNAs that regulate the function of flanking Hox transcription factors. We show that bi-directional transcription, temporal and spatial expression patterns and Hox regulatory function of the iab-4/iab-8 locus are conserved between fly and the beetle Tribolium castaneum. Computational predictions suggest iab-4 and iab-8 microRNAs can target common sites, and cell-culture assays confirm that iab-4 and iab-8 function overlaps on Hox target sites in both fly and beetle. However, we observe key differences in the way Hox genes are targeted. For instance, abd-A transcripts are targeted only by iab-8 in Drosophila, whereas both iab-4 and iab-8 bind to Tribolium abd-A. Our evolutionary and functional characterization of a bi-directionally transcribed microRNA establishes the iab-4/iab-8 system as a model for understanding how multiple products from sense and antisense microRNAs target common sites. © The Author(s) 2013.

U2 - 10.1093/nar/gks1445

DO - 10.1093/nar/gks1445

M3 - Article

C2 - 23335784

SN - 1362-4962

VL - 41

SP - 3352

EP - 3361

JO - Nucleic acids research.

JF - Nucleic acids research.

IS - 5

ER -

Structure, evolution and function of the bi-directionally transcribed iab-4/iab-8 microRNA locus in arthropods

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