Structure of an integrin-ligand complex deduced from solution X-ray scattering and site-directed mutagenesis

A. Paul Mould, Emlyn J H Symonds, Patrick A. Buckley, J. Günter Grossmann, Paul A. McEwan, Stephanie J. Barton, Janet A. Askari, Susan E. Craig, Jordi Bella, Martin J. Humphries

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The structural basis of the interaction of integrin heterodimers with their physiological ligands is poorly understood. We have used solution x-ray scattering to visualize the head region of integrin α5β 1 in an inactive (Ca2+-occupied) state, and in complex with a fragment of fibronectin containing the RGD and synergy recognition sequences. Shape reconstructions of the data have been interpreted in terms of appropriate molecular models. The scattering data suggest that the head region undergoes no gross conformational changes upon ligand binding but do lend support to a proposed outward movement of the hybrid domain in the β subunit. Fibronectin is observed to bind across the top of the head region, which contains an α subunit β-propeller and a β subunit vWF type A domain. The model of the complex indicates that the synergy region binds on the side of the β-propeller domain. In support of this suggestion, mutagenesis of a prominent loop region on the side of the propeller identifies two residues (Tyr208 and Ile210) involved in recognition of the synergy region. Our data provide the first view of a complex between an integrin and a macromolecular ligand in solution, at a nominal resolution of ∼10 Å.
    Original languageEnglish
    Pages (from-to)39993-39999
    Number of pages6
    JournalJournal of Biological Chemistry
    Volume278
    Issue number41
    DOIs
    Publication statusPublished - 10 Oct 2003

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