Studies on plasma iron in the rat. I. Investigations in vitro on iron binding

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    Abstract

    The binding of iron to protein in rat plasma was investigated using Fe59 as tracer. Paper electrophoresis studies showed that the iron was bound only to the β-globulin fraction and that none was bound to albumin at iron concentrations of 29-2860 μg./100 ml. plasma. The iron-protein complex was not precipitated either by 22% sodium sulfate solution or by 42% sodium sulfite solution, both reagents being commonly used for precipitating the globulins of human plasma. The complex was precipitated, however, by 60% saturation of the plasma with ammonium sulfate solution. Precipitation studies with this reagent indicated that the binding of iron to protein was maximal above pH 6.4 and fell rapidly below this value. © 1961.
    Original languageEnglish
    Pages (from-to)69-73
    Number of pages4
    JournalArchives of Biochemistry and Biophysics
    Volume92
    Issue number1
    Publication statusPublished - Jan 1961

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