Studies on plasmid stability, cell metabolism and superoxide dismutase production by Pgk- strains of Saccharomyces cerevisiae

M. A Z Ayub, S. Astolfi-Filho, F. Mavituna, S. G. Oliver

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A double mutant sod1/pgk1 strain of Saccharomyces cerevisiae has been constructed in order to investigate the effects of different environmental conditions on yeast physiology, plasmid stability, and superoxide dismutase (SOD) production. Strains were transformed with yeast episomal plasmids (YEp) containing both PGK1 and SOD1 genes and were grown on fermentable carbon sources and under vigorous aeration. Under these conditions, the presence of the PGK1 gene was made essential for growth and both genes were efficiently expressed. However, plasmid-borne PGK1 was found not to increase the stability of YEp vectors in batch cultures of Pgk- cells. Paradoxically, plasmid stability increased during the respiratory phase of growth. An investigation of the metabolism of Pgk- cells demonstrated that these glycolytic pathway mutants do not appreciably metabolize glycerol. Thus Pgk+, plasmid-containg, cells have a selective advantage during the respiratory phase of batch growth since they can utilize both glycerol and ethanol. © 1992 Springer-Verlag.
    Original languageEnglish
    Pages (from-to)615-620
    Number of pages5
    JournalApplied microbiology and biotechnology
    Volume37
    Issue number5
    DOIs
    Publication statusPublished - Aug 1992

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

    Fingerprint

    Dive into the research topics of 'Studies on plasmid stability, cell metabolism and superoxide dismutase production by Pgk- strains of Saccharomyces cerevisiae'. Together they form a unique fingerprint.

    Cite this