Studies on the exchange of valine-oxygen during the biosynthesis of δ-(L-α-Aminoadipoyl)-L-cysteinyl-D-valine.

J.E. Baldwin, R.M. Adlington, J.W. Bird, R.A. Field, N.M. O'Callaghan, C.J. Schofield

Research output: Contribution to journalArticlepeer-review


Incorporation of [4-2H6,18O2]-valine into δ-(L)-α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), by intact cells of Cephalosporium acremonium, demonstrated the intracellular exchange of one and both valine oxygen atoms. Incubation of [18O2]-valine with the purified ACV synthetase from C. acremonium gave exclusive incorporation of a single 18O label into ACV, consistent witheffectively non-reversible formation of a covalent valinoyl-ACV synthetase intermediate under in vitro conditions.
The fate of valine carboxyl-oxygen atoms during ACV biosynthesis has been studied, both in vivo and in vitro. Results demonstrate the loss of a single 18O label from [18O2]-valine during ACV formation.
Original languageUndefined
Pages (from-to)1099-1108
Number of pages10
Issue number6
Publication statusPublished - 1992


  • Cephalosporium acremonium
  • biosynthesis
  • δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine (ACV)
  • [18O2]-valine incorporation
  • penicillin

Research Beacons, Institutes and Platforms

  • Manchester Institute of Biotechnology

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