Study of osteoblasts mineralization in-vitro by Raman micro-spectroscopy.

Ioan Notingher, Julie E. Gough, Larry L. Hench

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The effect of bioinert and bioactive materials on the formation and mineralization of bone nodules in-vitro was studied by Raman spectroscopy. Human primary osteoblasts were cultured on bioinert fused silica and bioactive 45S5 Bioglass for 10 days. No mineralization was detected in both cases when the culture media was not supplemented with beta-glycerophosphate. When beta-glycerophosphate was added to the culture media, hydroxyapatite was detected in the Raman spectra for both substrates. However, when osteoblasts were cultured on fused silica, the intensity of HA peak was considerably lower compared to the values measured when the cells were cultured on 45S5 Bioglass. Bone nodules were obsd. only when 45S5 Bioglass was used as substrate and the mineralization was indicated by very intense HA peaks. [on SciFinder (R)]
    Original languageEnglish
    Pages (from-to)769-772
    JournalKey Engineering Materials
    Volume254-256
    DOIs
    Publication statusPublished - 2004

    Keywords

    • Bone (artificial; osteoblasts mineralization in-vitro by Raman micro-spectroscopy); Prosthetic materials and Prosthetics (bioactive glass; osteoblasts mineralization in-vitro by Raman micro-spectroscopy); Raman spectroscopy (microprobe; osteoblasts mineralization in-vitro by Raman micro-spectroscopy); Animal tissue culture; Human; Osteoblast (osteoblasts mineralization in-vitro by Raman micro-spectroscopy)

    Fingerprint

    Dive into the research topics of 'Study of osteoblasts mineralization in-vitro by Raman micro-spectroscopy.'. Together they form a unique fingerprint.

    Cite this