Subcellular fractionation evidence for a putative peroxisome-mitochondrion attachment in the liver of normal and genetically obese (ob/oband db/db) mice

V.M. Mann; V.U. Nwosu; A. Silcox; Carolyn Jones; K. Burdett; M.J. Connock

doi:10.1016/0305-0491(92)90047-U

Subcellular fractionation evidence for a putative peroxisome-mitochondrion attachment in the liver of normal and genetically obese (ob/oband db/db) mice

V.M. Mann, V.U. Nwosu, A. Silcox, Carolyn Jones, K. Burdett, M.J. Connock

Research output: Contribution to journal › Article › peer-review

Abstract

1. 1. Liver post-nuclear supernatants (PNS) from several mouse strains were fractionated by zonal centrifugation and fractions analysed by marker-enzyme estimations + electron microscopy. 2. 2. Rate-dependent banding of PNS yielded peroxisome-enriched (PER) and mitochondrion-enriched (MER) regions. 3. 3. Density-dependent banding of PER yielded peroxisomes (≈1.22 g/ml) well separated from mitochondria (≈1.8 g/ml). 4. 4. Density-dependent banding of MER yielded peroxisomes that co-distributed with mitochondria and electron microscopy revealed close proximity of the two organelles. 5. 5. Experiments demonstrated that co-distribution was not due to weak binding of proteins or to agglutination of organelles. 6. 6. The results indicate in vivo attachment of some mitochondria and peroxisomes. © 1992.

Original language	Undefined
Pages (from-to)	551-559
Number of pages	9
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	102
Issue number	3
DOIs	https://doi.org/10.1016/0305-0491(92)90047-U
Publication status	Published - 1992

Keywords

animal model
animal tissue
article
cell fractionation
centrifugation
electron microscopy
genetic disorder
liver
mitochondrion
mouse
nonhuman
obesity
peroxisome
priority journal, Animal
Biological Markers
Cell Fractionation
Centrifugation, Density Gradient
Female
Liver
Male
Mice
Mice, Obese
Microbodies
Microscopy, Electron
Mitochondria, Liver, Animalia

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10.1016/0305-0491(92)90047-U

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Cite this

Mann, V. M., Nwosu, V. U., Silcox, A., Jones, C., Burdett, K., & Connock, M. J. (1992). Subcellular fractionation evidence for a putative peroxisome-mitochondrion attachment in the liver of normal and genetically obese (ob/oband db/db) mice. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 102(3), 551-559. https://doi.org/10.1016/0305-0491(92)90047-U

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title = "Subcellular fractionation evidence for a putative peroxisome-mitochondrion attachment in the liver of normal and genetically obese (ob/oband db/db) mice",

abstract = "1. 1. Liver post-nuclear supernatants (PNS) from several mouse strains were fractionated by zonal centrifugation and fractions analysed by marker-enzyme estimations + electron microscopy. 2. 2. Rate-dependent banding of PNS yielded peroxisome-enriched (PER) and mitochondrion-enriched (MER) regions. 3. 3. Density-dependent banding of PER yielded peroxisomes (≈1.22 g/ml) well separated from mitochondria (≈1.8 g/ml). 4. 4. Density-dependent banding of MER yielded peroxisomes that co-distributed with mitochondria and electron microscopy revealed close proximity of the two organelles. 5. 5. Experiments demonstrated that co-distribution was not due to weak binding of proteins or to agglutination of organelles. 6. 6. The results indicate in vivo attachment of some mitochondria and peroxisomes. {\textcopyright} 1992.",

keywords = "animal model, animal tissue, article, cell fractionation, centrifugation, electron microscopy, genetic disorder, liver, mitochondrion, mouse, nonhuman, obesity, peroxisome, priority journal, Animal, Biological Markers, Cell Fractionation, Centrifugation, Density Gradient, Female, Liver, Male, Mice, Mice, Obese, Microbodies, Microscopy, Electron, Mitochondria, Liver, Animalia",

author = "V.M. Mann and V.U. Nwosu and A. Silcox and Carolyn Jones and K. Burdett and M.J. Connock",

note = "cited By 4",

year = "1992",

doi = "10.1016/0305-0491(92)90047-U",

language = "Undefined",

volume = "102",

pages = "551--559",

journal = "Comparative Biochemistry and Physiology -- Part B: Biochemistry and",

issn = "0305-0491",

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Mann, VM, Nwosu, VU, Silcox, A, Jones, C, Burdett, K & Connock, MJ 1992, 'Subcellular fractionation evidence for a putative peroxisome-mitochondrion attachment in the liver of normal and genetically obese (ob/oband db/db) mice', Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 102, no. 3, pp. 551-559. https://doi.org/10.1016/0305-0491(92)90047-U

Subcellular fractionation evidence for a putative peroxisome-mitochondrion attachment in the liver of normal and genetically obese (ob/oband db/db) mice. / Mann, V.M.; Nwosu, V.U.; Silcox, A. et al.
In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 102, No. 3, 1992, p. 551-559.

Research output: Contribution to journal › Article › peer-review

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AU - Mann, V.M.

AU - Nwosu, V.U.

AU - Silcox, A.

AU - Jones, Carolyn

AU - Burdett, K.

AU - Connock, M.J.

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N2 - 1. 1. Liver post-nuclear supernatants (PNS) from several mouse strains were fractionated by zonal centrifugation and fractions analysed by marker-enzyme estimations + electron microscopy. 2. 2. Rate-dependent banding of PNS yielded peroxisome-enriched (PER) and mitochondrion-enriched (MER) regions. 3. 3. Density-dependent banding of PER yielded peroxisomes (≈1.22 g/ml) well separated from mitochondria (≈1.8 g/ml). 4. 4. Density-dependent banding of MER yielded peroxisomes that co-distributed with mitochondria and electron microscopy revealed close proximity of the two organelles. 5. 5. Experiments demonstrated that co-distribution was not due to weak binding of proteins or to agglutination of organelles. 6. 6. The results indicate in vivo attachment of some mitochondria and peroxisomes. © 1992.

AB - 1. 1. Liver post-nuclear supernatants (PNS) from several mouse strains were fractionated by zonal centrifugation and fractions analysed by marker-enzyme estimations + electron microscopy. 2. 2. Rate-dependent banding of PNS yielded peroxisome-enriched (PER) and mitochondrion-enriched (MER) regions. 3. 3. Density-dependent banding of PER yielded peroxisomes (≈1.22 g/ml) well separated from mitochondria (≈1.8 g/ml). 4. 4. Density-dependent banding of MER yielded peroxisomes that co-distributed with mitochondria and electron microscopy revealed close proximity of the two organelles. 5. 5. Experiments demonstrated that co-distribution was not due to weak binding of proteins or to agglutination of organelles. 6. 6. The results indicate in vivo attachment of some mitochondria and peroxisomes. © 1992.

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KW - Female

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U2 - 10.1016/0305-0491(92)90047-U

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M3 - Article

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JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and

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