Super agonist actions of clothianidin and related compounds on the SADβ2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes

Makoto Ihara; Kazuhiko Matsuda; Masaru Shimomura; David B. Sattelle; Koichiro Komai

doi:10.1271/bbb.68.761

Super agonist actions of clothianidin and related compounds on the SADβ2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes

Makoto Ihara, Kazuhiko Matsuda, Masaru Shimomura, David B. Sattelle, Koichiro Komai

Research output: Contribution to journal › Article › peer-review

Abstract

To compare the actions of clothianidin, a neonicotinoid acting on insect nicotinic acetylcholine receptors, and related compounds with that of imidacloprid, the compounds were tested on the Drosophila SAD-chicken β2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes using two-electrode voltage-clamp electro-physiology. The maximum response of the SADβ2 nicotinic receptor to clothianidin was larger than that observed for acetylcholine. Ring breakage of the imidazolidine ring of imidacloprid resulting in the generation of a guanidine group was critical for this super agonist action.

Original language	English
Pages (from-to)	761-763
Number of pages	2
Journal	Bioscience, Biotechnology and Biochemistry
Volume	68
Issue number	3
DOIs	https://doi.org/10.1271/bbb.68.761
Publication status	Published - Mar 2004

Keywords

Clothianidin
Drosophila melanogaster SAD-chicken β2 hybrid nicotinic receptor
Imidacloprid
Neonicotinoid
Nicotinic acetylcholine receptor

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10.1271/bbb.68.761

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title = "Super agonist actions of clothianidin and related compounds on the SADβ2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes",

abstract = "To compare the actions of clothianidin, a neonicotinoid acting on insect nicotinic acetylcholine receptors, and related compounds with that of imidacloprid, the compounds were tested on the Drosophila SAD-chicken β2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes using two-electrode voltage-clamp electro-physiology. The maximum response of the SADβ2 nicotinic receptor to clothianidin was larger than that observed for acetylcholine. Ring breakage of the imidazolidine ring of imidacloprid resulting in the generation of a guanidine group was critical for this super agonist action.",

keywords = "Clothianidin, Drosophila melanogaster SAD-chicken β2 hybrid nicotinic receptor, Imidacloprid, Neonicotinoid, Nicotinic acetylcholine receptor",

author = "Makoto Ihara and Kazuhiko Matsuda and Masaru Shimomura and Sattelle, {David B.} and Koichiro Komai",

year = "2004",

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doi = "10.1271/bbb.68.761",

language = "English",

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pages = "761--763",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "1347-6947",

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TY - JOUR

T1 - Super agonist actions of clothianidin and related compounds on the SADβ2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes

AU - Ihara, Makoto

AU - Matsuda, Kazuhiko

AU - Shimomura, Masaru

AU - Sattelle, David B.

AU - Komai, Koichiro

PY - 2004/3

Y1 - 2004/3

N2 - To compare the actions of clothianidin, a neonicotinoid acting on insect nicotinic acetylcholine receptors, and related compounds with that of imidacloprid, the compounds were tested on the Drosophila SAD-chicken β2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes using two-electrode voltage-clamp electro-physiology. The maximum response of the SADβ2 nicotinic receptor to clothianidin was larger than that observed for acetylcholine. Ring breakage of the imidazolidine ring of imidacloprid resulting in the generation of a guanidine group was critical for this super agonist action.

AB - To compare the actions of clothianidin, a neonicotinoid acting on insect nicotinic acetylcholine receptors, and related compounds with that of imidacloprid, the compounds were tested on the Drosophila SAD-chicken β2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes using two-electrode voltage-clamp electro-physiology. The maximum response of the SADβ2 nicotinic receptor to clothianidin was larger than that observed for acetylcholine. Ring breakage of the imidazolidine ring of imidacloprid resulting in the generation of a guanidine group was critical for this super agonist action.

KW - Clothianidin

KW - Drosophila melanogaster SAD-chicken β2 hybrid nicotinic receptor

KW - Imidacloprid

KW - Neonicotinoid

KW - Nicotinic acetylcholine receptor

U2 - 10.1271/bbb.68.761

DO - 10.1271/bbb.68.761

M3 - Article

C2 - 15056916

SN - 1347-6947

VL - 68

SP - 761

EP - 763

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 3

ER -

Super agonist actions of clothianidin and related compounds on the SADβ2 nicotinic acetylcholine receptor expressed in Xenopus laevis oocytes

Abstract

Keywords

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