Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy

Maobin Xie, Dejun Fan, Yi Li, Xiaowen He, Xiaoming Chen, Yufeng Chen, Jixiang Zhu, Guibin Xu, Xiaojian Wu, Ping Lan

    Research output: Contribution to journalArticlepeer-review


    Purpose: To deliver insoluble natural compounds into colon cancer cells in a controlled fashion. Materials and methods: Curcumin (CM)–silk fibroin (SF) nanoparticles (NPs) were prepared by solution-enhanced dispersion by supercritical CO2 (SEDS) (20 MPa pressure, 1:2 CM:SF ratio, 1% concentration), and their physicochemical properties, intracellular uptake efficiency, in vitro anticancer effect, toxicity, and mechanisms were evaluated and analyzed. Results: CM-SF NPs (<100 nm) with controllable particle size were prepared by SEDS. CM-SF NPs had a time-dependent intracellular uptake ability, which led to an improved inhibition effect on colon cancer cells. Interestingly, the anticancer effect of CM-SF NPs was improved, while the side effect on normal human colon mucosal epithelial cells was reduced by a concentration of ~10 μg/mL. The anticancer mechanism involves cell-cycle arrest in the G0/G1 and G2/M phases in association with inducing apoptotic cells. Conclusion: The natural compound-loaded SF nanoplatform prepared by SEDS indicates promising colon cancer-therapy potential.

    Original languageEnglish
    Pages (from-to)7751-7761
    Number of pages11
    JournalInternational Journal of Nanomedicine
    Publication statusPublished - 20 Oct 2017


    • Colon cancer
    • Nanoparticle
    • Natural compounds
    • Silk fibroin
    • Supercritical


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