Sustained glycolytic oscillations in individual isolated yeast cells

Anna Karin Gustavsson, David D. Van Niekerk, Caroline B. Adiels, Franco B. Du Preez, Mattias Goksör, Jacky L. Snoep

    Research output: Contribution to journalArticlepeer-review


    Yeast glycolytic oscillations have been studied since the 1950s in cell-free extracts and intact cells. For intact cells, sustained oscillations have so far only been observed at the population level, i.e. for synchronized cultures at high biomass concentrations. Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simulated the heterogeneity in the response of the individual cells, assuming small differences in a single internal parameter. This is the first time that sustained limit-cycle oscillations have been demonstrated in isolated yeast cells. © 2012 FEBS.
    Original languageEnglish
    Pages (from-to)2837-2847
    Number of pages10
    JournalFEBS Journal
    Issue number16
    Publication statusPublished - Aug 2012


    • cell heterogeneity
    • glycolysis
    • limit-cycle oscillation
    • mathematical model
    • microfluidic chamber


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