Synthesis of [4S-2H]NADH, [4R-2H]NADH, [4-2H2]NADH and [4-2H]NAD+ cofactors through heterogeneous biocatalysis in heavy water

Jack Rowbotham, Adam Hardy, Holly Reeve, Kylie A. Vincent

Research output: Contribution to journalArticlepeer-review


This practitioner protocol describes the synthesis of a family of deuterated nicotinamide cofactors: [4S- 2H]NADH, [4R- 2H]NADH, [4- 2H 2]NADH and [4- 2H]NAD +. The application of a recently developed H 2-driven heterogeneous biocatalyst enables the cofactors to be prepared with high (>90%) 2H-incorporation with 2H 2O as the only isotope source.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Issue number4
Early online date26 Jan 2021
Publication statusPublished - 1 Apr 2021


  • D O
  • H O
  • deuterated cofactor
  • dihydrogen gas (H )
  • immobilised enzymes

Research Beacons, Institutes and Platforms

  • Manchester Institute of Biotechnology


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