Synthetic heparan sulfate dodecasaccharides reveal single sulfation site interconverts CXCL8 and CXCL12 chemokine biology

Gordon C Jayson; Steen U Hansen; Gavin J Miller; Claire L Cole; Graham Rushton; Egle Avizienyte; John M Gardiner

doi:10.1039/C5CC05222J

Synthetic heparan sulfate dodecasaccharides reveal single sulfation site interconverts CXCL8 and CXCL12 chemokine biology

Gordon C Jayson, Steen U Hansen, Gavin J Miller, Claire L Cole, Graham Rushton, Egle Avizienyte, John M Gardiner

Research output: Contribution to journal › Article › peer-review

Abstract

The multigram-scale synthesis of a sulfation-site programmed heparin-like dodecasaccharide is described. Evaluation alongside dodecasaccharides lacking this single glucosamine O6-sulfation, or having per-O6-sulfation, shows that site-specific modification of the terminal glucosamine dramatically interconverts regulation of in vitro and in vivo biology mediated by the two important chemokines, CXCL12 (SDF1α) or CXCL8 (IL-8).

Original language	English
Pages (from-to)	13846-9
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	72
DOIs	https://doi.org/10.1039/C5CC05222J
Publication status	Published - 18 Sept 2015

Keywords

Carbohydrate Sequence
Cell Migration Assays, Leukocyte
Chemokine CXCL12
Heparitin Sulfate
Human Umbilical Vein Endothelial Cells
Humans
Interleukin-8
Leukocytes
Oligosaccharides

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title = "Synthetic heparan sulfate dodecasaccharides reveal single sulfation site interconverts CXCL8 and CXCL12 chemokine biology",

abstract = "The multigram-scale synthesis of a sulfation-site programmed heparin-like dodecasaccharide is described. Evaluation alongside dodecasaccharides lacking this single glucosamine O6-sulfation, or having per-O6-sulfation, shows that site-specific modification of the terminal glucosamine dramatically interconverts regulation of in vitro and in vivo biology mediated by the two important chemokines, CXCL12 (SDF1α) or CXCL8 (IL-8).",

keywords = "Carbohydrate Sequence, Cell Migration Assays, Leukocyte, Chemokine CXCL12, Heparitin Sulfate, Human Umbilical Vein Endothelial Cells, Humans, Interleukin-8, Leukocytes, Oligosaccharides",

author = "Jayson, {Gordon C} and Hansen, {Steen U} and Miller, {Gavin J} and Cole, {Claire L} and Graham Rushton and Egle Avizienyte and Gardiner, {John M}",

note = "MRC and CRUK acknowledgedOpen Access SI file",

year = "2015",

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day = "18",

doi = "10.1039/C5CC05222J",

language = "English",

volume = "51",

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journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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T1 - Synthetic heparan sulfate dodecasaccharides reveal single sulfation site interconverts CXCL8 and CXCL12 chemokine biology

AU - Jayson, Gordon C

AU - Hansen, Steen U

AU - Miller, Gavin J

AU - Cole, Claire L

AU - Rushton, Graham

AU - Avizienyte, Egle

AU - Gardiner, John M

N1 - MRC and CRUK acknowledgedOpen Access SI file

PY - 2015/9/18

Y1 - 2015/9/18

N2 - The multigram-scale synthesis of a sulfation-site programmed heparin-like dodecasaccharide is described. Evaluation alongside dodecasaccharides lacking this single glucosamine O6-sulfation, or having per-O6-sulfation, shows that site-specific modification of the terminal glucosamine dramatically interconverts regulation of in vitro and in vivo biology mediated by the two important chemokines, CXCL12 (SDF1α) or CXCL8 (IL-8).

AB - The multigram-scale synthesis of a sulfation-site programmed heparin-like dodecasaccharide is described. Evaluation alongside dodecasaccharides lacking this single glucosamine O6-sulfation, or having per-O6-sulfation, shows that site-specific modification of the terminal glucosamine dramatically interconverts regulation of in vitro and in vivo biology mediated by the two important chemokines, CXCL12 (SDF1α) or CXCL8 (IL-8).

KW - Carbohydrate Sequence

KW - Cell Migration Assays, Leukocyte

KW - Chemokine CXCL12

KW - Heparitin Sulfate

KW - Human Umbilical Vein Endothelial Cells

KW - Humans

KW - Interleukin-8

KW - Leukocytes

KW - Oligosaccharides

U2 - 10.1039/C5CC05222J

DO - 10.1039/C5CC05222J

M3 - Article

SN - 1364-548X

VL - 51

SP - 13846

EP - 13849

JO - Chemical Communications

JF - Chemical Communications

IS - 72

ER -

Synthetic heparan sulfate dodecasaccharides reveal single sulfation site interconverts CXCL8 and CXCL12 chemokine biology

Abstract

Keywords

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