Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome.

Jonathan Worboys; John Sinclair; Yinyin Yuan; Claus Jorgensen

doi:10.1038/nmeth.3072

Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome.

Jonathan Worboys, John Sinclair, Yinyin Yuan, Claus Jorgensen

Research output: Contribution to journal › Article › peer-review

Abstract

In targeted proteomics it is critical that peptides are not only proteotypic but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. We show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties. We applied this technique to identify quantotypic peptides for 21% of the human kinome.

Original language	English
Pages (from-to)	1041–1044
Journal	Nature Methods
Volume	11
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.3072
Publication status	Published - 24 Aug 2014

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Manchester Cancer Research Centre

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10.1038/nmeth.3072

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abstract = "In targeted proteomics it is critical that peptides are not only proteotypic but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. We show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties. We applied this technique to identify quantotypic peptides for 21% of the human kinome.",

author = "Jonathan Worboys and John Sinclair and Yinyin Yuan and Claus Jorgensen",

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AB - In targeted proteomics it is critical that peptides are not only proteotypic but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. We show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties. We applied this technique to identify quantotypic peptides for 21% of the human kinome.

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Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome.

Abstract

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