T-regulatory cell phenotypes in peripheral blood and bronchoalveolar lavage from non-asthmatic and asthmatic subjects.

Adrian J Baatjes; Steven G Smith; Rick Watson; Karen Howie; Desmond Murphy; Mark Larché; Judah A Denburg; Mark D Inman; Paul M O'Byrne

doi:10.1111/cea.12594

T-regulatory cell phenotypes in peripheral blood and bronchoalveolar lavage from non-asthmatic and asthmatic subjects.

Adrian J Baatjes, Steven G Smith, Rick Watson, Karen Howie, Desmond Murphy, Mark Larché, Judah A Denburg, Mark D Inman, Paul M O'Byrne

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: An unresolved issue in T regulatory (Treg) cell biology is the lack of consensus on phenotypic markers that accurately define the natural Treg (nTreg) population. OBJECTIVES: To examine nTreg frequency and functional capacity in healthy controls, and their frequency in asthmatic subjects using three different phenotypic strategies. We hypothesized that phenotypically different nTreg are quantitatively and functionally different. METHODS: 34 healthy, non-asthmatic and 17 asthmatic subjects were studied. Three nTreg phenotypes were defined: nTreg1 (CD4(+) CD25(+) Foxp3(+) ), nTreg2 (CD4(+) CD25(+) CD127(low) Foxp3(+) ), and nTreg3 (CD4(+) CD25(high) Foxp3(+) ). The flow cytometric determination of nTreg frequency in peripheral blood (PB) and bronchoalveolar lavage (BAL) was performed using fluorescently labelled antibodies. PB nTreg functional capacity was assessed using a CFSE-based suppression assay. RESULTS: There was a significantly lower frequency of peripheral blood nTreg3 compared to nTreg2 and nTreg1 (p

Original language	English
Journal	Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
DOIs	https://doi.org/10.1111/cea.12594
Publication status	Published - 14 Jul 2015

Keywords

BAL
Foxp3
T regulatory cell
asthma
flow cytometry
peripheral blood
suppression assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Baatjes, A. J., Smith, S. G., Watson, R., Howie, K., Murphy, D., Larché, M., Denburg, J. A., Inman, M. D., & O'Byrne, P. M. (2015). T-regulatory cell phenotypes in peripheral blood and bronchoalveolar lavage from non-asthmatic and asthmatic subjects. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. https://doi.org/10.1111/cea.12594

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title = "T-regulatory cell phenotypes in peripheral blood and bronchoalveolar lavage from non-asthmatic and asthmatic subjects.",

abstract = "BACKGROUND: An unresolved issue in T regulatory (Treg) cell biology is the lack of consensus on phenotypic markers that accurately define the natural Treg (nTreg) population. OBJECTIVES: To examine nTreg frequency and functional capacity in healthy controls, and their frequency in asthmatic subjects using three different phenotypic strategies. We hypothesized that phenotypically different nTreg are quantitatively and functionally different. METHODS: 34 healthy, non-asthmatic and 17 asthmatic subjects were studied. Three nTreg phenotypes were defined: nTreg1 (CD4(+) CD25(+) Foxp3(+) ), nTreg2 (CD4(+) CD25(+) CD127(low) Foxp3(+) ), and nTreg3 (CD4(+) CD25(high) Foxp3(+) ). The flow cytometric determination of nTreg frequency in peripheral blood (PB) and bronchoalveolar lavage (BAL) was performed using fluorescently labelled antibodies. PB nTreg functional capacity was assessed using a CFSE-based suppression assay. RESULTS: There was a significantly lower frequency of peripheral blood nTreg3 compared to nTreg2 and nTreg1 (p",

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doi = "10.1111/cea.12594",

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Baatjes, AJ, Smith, SG, Watson, R, Howie, K, Murphy, D, Larché, M, Denburg, JA, Inman, MD & O'Byrne, PM 2015, 'T-regulatory cell phenotypes in peripheral blood and bronchoalveolar lavage from non-asthmatic and asthmatic subjects.', Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. https://doi.org/10.1111/cea.12594

TY - JOUR

T1 - T-regulatory cell phenotypes in peripheral blood and bronchoalveolar lavage from non-asthmatic and asthmatic subjects.

AU - Baatjes, Adrian J

AU - Smith, Steven G

AU - Watson, Rick

AU - Howie, Karen

AU - Murphy, Desmond

AU - Larché, Mark

AU - Denburg, Judah A

AU - Inman, Mark D

AU - O'Byrne, Paul M

PY - 2015/7/14

Y1 - 2015/7/14

N2 - BACKGROUND: An unresolved issue in T regulatory (Treg) cell biology is the lack of consensus on phenotypic markers that accurately define the natural Treg (nTreg) population. OBJECTIVES: To examine nTreg frequency and functional capacity in healthy controls, and their frequency in asthmatic subjects using three different phenotypic strategies. We hypothesized that phenotypically different nTreg are quantitatively and functionally different. METHODS: 34 healthy, non-asthmatic and 17 asthmatic subjects were studied. Three nTreg phenotypes were defined: nTreg1 (CD4(+) CD25(+) Foxp3(+) ), nTreg2 (CD4(+) CD25(+) CD127(low) Foxp3(+) ), and nTreg3 (CD4(+) CD25(high) Foxp3(+) ). The flow cytometric determination of nTreg frequency in peripheral blood (PB) and bronchoalveolar lavage (BAL) was performed using fluorescently labelled antibodies. PB nTreg functional capacity was assessed using a CFSE-based suppression assay. RESULTS: There was a significantly lower frequency of peripheral blood nTreg3 compared to nTreg2 and nTreg1 (p

AB - BACKGROUND: An unresolved issue in T regulatory (Treg) cell biology is the lack of consensus on phenotypic markers that accurately define the natural Treg (nTreg) population. OBJECTIVES: To examine nTreg frequency and functional capacity in healthy controls, and their frequency in asthmatic subjects using three different phenotypic strategies. We hypothesized that phenotypically different nTreg are quantitatively and functionally different. METHODS: 34 healthy, non-asthmatic and 17 asthmatic subjects were studied. Three nTreg phenotypes were defined: nTreg1 (CD4(+) CD25(+) Foxp3(+) ), nTreg2 (CD4(+) CD25(+) CD127(low) Foxp3(+) ), and nTreg3 (CD4(+) CD25(high) Foxp3(+) ). The flow cytometric determination of nTreg frequency in peripheral blood (PB) and bronchoalveolar lavage (BAL) was performed using fluorescently labelled antibodies. PB nTreg functional capacity was assessed using a CFSE-based suppression assay. RESULTS: There was a significantly lower frequency of peripheral blood nTreg3 compared to nTreg2 and nTreg1 (p

KW - BAL

KW - Foxp3

KW - T regulatory cell

KW - asthma

KW - flow cytometry

KW - peripheral blood

KW - suppression assay

U2 - 10.1111/cea.12594

DO - 10.1111/cea.12594

M3 - Article

C2 - 26177872

SN - 1365-2222

JO - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

ER -

T-regulatory cell phenotypes in peripheral blood and bronchoalveolar lavage from non-asthmatic and asthmatic subjects.

Abstract

Keywords

UN SDGs

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