Tail-anchored protein insertion into yeast ER requires a novel posttranslational mechanism which is independent of the SEC machinery

Gregor J. Steel, Judy Brownsword, Colin J. Stirling

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Tail-anchored or C-terminally-anchored proteins play many essential roles in eukaryotic cells. However, targeting and insertion of this class of membrane protein has remained elusive. In this study, we reconstitute insertion of tail-anchored proteins into microsomes derived from Saccharomyces cerevisiae. Using this approach, we are able to genetically manipulate the composition of the microsomes in order to address the question of which components of the endoplasmic reticulum (ER) are required for this process. We show that tail-anchored protein insertion is not dependent on the classical SEC translocation machinery but rather occurs via an ATP-dependent pathway involving at least one novel membrane protein factor. We further demonstrate that the specificity of this pathway is conserved between yeast and mammals.
    Original languageEnglish
    Pages (from-to)11914-11920
    Number of pages6
    JournalBiochemistry
    Volume41
    Issue number39
    DOIs
    Publication statusPublished - 1 Oct 2002

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